特异性Western Blot: Specific for the alpha form (in human and rat) and this antibody reacts with GSTs of the alpha class, with minimal cross-reaction with mu class GSTs, and no cross-reaction with pi class GSTs. Reactive with > 0.2 µg of recombinant human GST A1-1.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: 1/1000. Predicted molecular weight: 25 kDa. A dilution of 1/1000 for Western blots will yield a positive result with > 0.2 µg of recombinant human A1-1 GST.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
序列相似性Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
结构域The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.