Glutathione Reductase (GR) Assay试剂盒(ab83461)

概述

  • 产品名称
    Glutathione Reductase (GR) Assay试剂盒
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • 检测类型
    Enzyme activity
  • 灵敏度
    > 0.1 mU/ml
  • 范围
    0.1 mU/ml - 40 mU/ml
  • 检测时间
    0h 40m
  • 产品概述

    Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab83461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

  • Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

  • Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

  • Glutathione Reductase assay time line using ab83461.
  • TNB Standard Curve using ab83461.

实验方案

文献

This product has been referenced in:
  • Cao Z  et al. Isochlorogenic acid C prevents enterovirus 71 infection via modulating redox homeostasis of glutathione. Sci Rep 7:16278 (2017). Read more (PubMed: 29176678) »
  • Choi S  et al. Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments. PLoS One 11:e0167391 (2016). Read more (PubMed: 27907194) »

See all 2 Publications for this product

客户评价及客户问答

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The procedure is given in protocol booklet.

http://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

Please thaw frozen tissue on ...

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Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use ar...

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Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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