使用敲除细胞株进行验证RabMAb

Anti-Glutathione Reductase抗体[EPR7238] (ab124995)

概述

  • 产品名称Anti-Glutathione Reductase抗体[EPR7238]
    参阅全部 Glutathione Reductase 一抗
  • 描述
    兔单克隆抗体[EPR7238] to Glutathione Reductase
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Glutathione Reductase.

  • 阳性对照
    • Jurkat, HeLa, A549 and Caco-2 cell lysates; permeabilized HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    Anti-Glutathione Reductase antibody [EPR7238] 图像



    • Predicted band size : 56 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab124995 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124995 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE.  Ab124995 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 56 kDa
    • ab124995, at a 1/10 dilution, staining Glutathione Reductase in permeabilized HeLa cells by Flow Cytometry (red). Analysis with a rabbit IgG (negative control) is shown in green.

    Anti-Glutathione Reductase antibody [EPR7238] (ab124995)参考文献

    ab124995 has not yet been referenced specifically in any publications.

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