Anti-Glutathione Reductase抗体(ab87330)

概述

  • 产品名称Anti-Glutathione Reductase抗体
    参阅全部 Glutathione Reductase 一抗
  • 描述
    兔多克隆抗体to Glutathione Reductase
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Marmoset (common)
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Glutathione Reductase.

    (Peptide available as ab95921.)

  • 阳性对照
    • This antibody gave a positive signal in HeLa and Jurkat whole cell lysates and in the following human tissue lysates: Brain; Liver; Testis; Lung; Heart.

性能

应用

Our Abpromise guarantee covers the use of ab87330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).

靶标

  • 功能Maintains high levels of reduced glutathione in the cytosol.
  • 序列相似性Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • 结构域Each subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface.
  • 细胞定位Cytoplasm and Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GLR1 antibody
    • GLUR antibody
    • Glutathione reductase antibody
    • Glutathione reductase mitochondrial antibody
    • Glutathione reductase, mitochondrial antibody
    • GR antibody
    • Gr1 antibody
    • GRase antibody
    • GRD 1 antibody
    • GRD1 antibody
    • GSHR_HUMAN antibody
    • GSR antibody
    • MGC78522 antibody
    see all

Anti-Glutathione Reductase antibody 图像

  • All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)
    Lane 6 : Lung (Human) Tissue Lysate
    Lane 7 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 37 kDa,50 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

Anti-Glutathione Reductase antibody (ab87330)参考文献

ab87330 has not yet been referenced specifically in any publications.

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