Glutathione Peroxidase Assay试剂盒(Colorimetric) (ab102530)

概述

  • 产品名称Glutathione Peroxidase Assay试剂盒(Colorimetric)
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Platelets, Other biological fluids, Tissue Extracts
  • 检测类型Enzyme activity
  • 灵敏度
    0.5 mU/ml
  • 检测时间
    0h 40m
  • 种属反应性
    与反应: Mouse, Rat, Human, all Mammals
  • 产品概述

    Abcam's Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530) is an assay where glutathione peroxidase (GPx) reduces the probe Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by glutathione reductase (GR). The decrease of NADPH (easily measured at OD=340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    The Glutathione Peroxidase (GPx, EC 1.11.1.9) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Glutathione Peroxidase (GPx, EC 1.11.1.9) is an enzyme family with peroxidase activity, and plays important role in protecting of organisms from oxidative damage. It converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols, or reduce free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.

应用

Our Abpromise guarantee covers the use of ab102530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Glutathione Peroxidase Assay Kit (Colorimetric) 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Glutathione Peroxidase Activity measured in biological fluids showing quantity (nmol) per 1 mL after 10 min of incubation. Samples diluted 1-27 fold.

  • Glutathione Peroxidase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 10 min of incubation. Undiluted sample contained 2.5e6 cells per well. Samples were diluted 1-9 fold.

  • Tests example obtained using ab102530

实验方案

Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530)参考文献

This product has been referenced in:
  • Ann JY  et al. Mulberry leaves (Morus alba L.) ameliorate obesity-induced hepatic lipogenesis, fibrosis, and oxidative stress in high-fat diet-fed mice. Genes Nutr 10:46 (2015). Mouse . Read more (PubMed: 26463593) »
  • Geraghty P  et al. The glutathione peroxidase 1-protein tyrosine phosphatase 1B-protein phosphatase 2A axis. A key determinant of airway inflammation and alveolar destruction. Am J Respir Cell Mol Biol 49:721-30 (2013). Mouse . Read more (PubMed: 23590304) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry.

I am happy to confirm that ab102530 (Glutathione Peroxidase Assay Kit) is suitable for all mammalian samples, including rat samples.

I wish your customer good luck with their experiments.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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I have recieved a reply from the lab who confirm that there was a problem with the positive control from this lot. Unfortunately the positive control from this lot lost activity for unforeseen reasons
I am sorry this product did not perform as sta...

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I am currently in contact with the lab who are rechecking the positive control from this lot.
In the meantime, they would like to know what difference you got in the absorbance from the vector alone and the GPx ex...

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I am sorry to confirm that we do not offer the positive control separately for purchase.

I am sorry to not have a positive answer on this occasion and wish you a good weekend.

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Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use ar...

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Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

I will be looking forward to hearing from you soon.

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Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that sto...

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Please do not get confused with cytosol and mitochondrial SODs for plasma; the information I had provided was for general cells samples. I had missed the fact you are going to use plasma and plasma does not contain...

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I can confirm that the kit ab65354 will detect all SODs (Cu/Zn, Mn and FeSOD).

If it is desired to measure SOD activity from cytosol and mitochondria separately,these can be separated by using ab65320 Mito...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"