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Abcam's Glutathione Peroxidase Assay Kit (Colorimetric) (ab102530) is an assay where glutathione peroxidase (GPx) reduces the probe Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by glutathione reductase (GR). The decrease of NADPH (easily measured at OD=340 nm) is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.
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The Glutathione Peroxidase (GPx, EC 184.108.40.206) family of enzymes plays and important role in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG) while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders.
|Cumene Hydroperoxide||Yellow||1 vial|
|Glutathione Reductase||Green||1 vial|
|GPx Assay Buffer||WM||1 x 25ml|
|GPx Positive Control||Red||1 vial|
Our Abpromise guarantee covers the use of ab102530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Glutathione Peroxidase Activity measured in biological fluids showing quantity (nmol) per 1 mL after 10 min of incubation. Samples diluted 1-27 fold.
Glutathione Peroxidase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 10 min of incubation. Undiluted sample contained 2.5e6 cells per well. Samples were diluted 1-9 fold.