The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).
功能Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
组织特异性Present primarily in testis.
序列相似性Belongs to the glutathione peroxidase family.
ab51944 at 0.3ug/ml staining Glutathione peroxidase 4 in human testis tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.