Anti-Glutathione Peroxidase 3抗体[23B1] (ab27325)

概述

  • 产品名称Anti-Glutathione Peroxidase 3抗体[23B1]
    参阅全部 Glutathione Peroxidase 3 一抗
  • 描述
    小鼠单克隆抗体[23B1] to Glutathione Peroxidase 3
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    His-tagged recombinant human Glutathione peroxidase 3 protein purified from E. coli.

  • 阳性对照
    • Bosc23 cell lysate transfected with myc-Glutathione Peroxidase 3.

性能

应用

Our Abpromise guarantee covers the use of ab27325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500. Predicted molecular weight: 25 kDa.

靶标

  • 功能Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
  • 组织特异性Secreted in plasma.
  • 序列相似性Belongs to the glutathione peroxidase family.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Extracellular glutathione peroxidase antibody
    • Glutathione peroxidase 3 (plasma) antibody
    • Glutathione peroxidase 3 antibody
    • GPX 3 antibody
    • GPx P antibody
    • GPx-3 antibody
    • GPx-P antibody
    • GPX3 antibody
    • GPX3_HUMAN antibody
    • GPXP antibody
    • GSHPx 3 antibody
    • GSHPx P antibody
    • GSHPx-3 antibody
    • GSHPx-P antibody
    • Plasma glutathione peroxidase 3 antibody
    • Plasma glutathione peroxidase antibody
    see all

Anti-Glutathione Peroxidase 3 antibody [23B1] 图像

  • All lanes : Anti-Glutathione Peroxidase 3 antibody [23B1] (ab27325)

    Lane 1 : Bosc23 cell lysates
    Lane 2 : Bosc23 cell lysate transfected with myc-Glutathione Peroxidase 3


    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab27325 (4µg/ml) staining Glutathione Peroxidase 3 in human renal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staininig of renal tubules.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab27325 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27325, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glutathione Peroxidase 3 antibody [23B1] (ab27325)参考文献

This product has been referenced in:
  • Chang SN  et al. Glutathione Peroxidase 3 Inhibits Prostate Tumorigenesis in TRAMP Mice. Prostate 76:1387-98 (2016). Read more (PubMed: 27325372) »
  • Chang SN  et al. High animal fat intake enhances prostate cancer progression and reduces glutathione peroxidase 3 expression in early stages of TRAMP mice. Prostate 74:1266-77 (2014). WB, IHC-P ; Human . Read more (PubMed: 25053105) »

See all 7 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab27325.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"