Anti-Glutathione Peroxidase 2抗体(ab64322)

概述

  • 产品名称Anti-Glutathione Peroxidase 2抗体
    参阅全部 Glutathione Peroxidase 2 一抗
  • 描述
    兔多克隆抗体to Glutathione Peroxidase 2
  • 特异性Reacts specifically with human 22 kDa Glutathione Peroxidase 2 protein
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from C terminal domain of human Glutathione Peroxidase 2 protein

性能

应用

Our Abpromise guarantee covers the use of ab64322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/5000. Predicted molecular weight: 21 kDa.
IHC-P 1/1000.

靶标

  • 功能Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors.
  • 组织特异性Mostly in liver and gastrointestinal tract, not found in heart or kidney.
  • 序列相似性Belongs to the glutathione peroxidase family.
  • 细胞定位Cytoplasm. Mainly cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gastrointestinal glutathione peroxidase antibody
    • GI GPx antibody
    • Glutathione peroxidase 2 (gastrointestinal) antibody
    • Glutathione peroxidase 2 antibody
    • Glutathione peroxidase gastrointestinal antibody
    • Glutathione peroxidase related protein 2 antibody
    • Glutathione peroxidase-gastrointestinal antibody
    • Glutathione peroxidase-related protein 2 antibody
    • GPRP antibody
    • GPRP-2 antibody
    • GPx 2 antibody
    • GPx-2 antibody
    • GPx-GI antibody
    • GPX2 antibody
    • GPX2_HUMAN antibody
    • GSHPx 2 antibody
    • GSHPx GI antibody
    • GSHPx-2 antibody
    • GSHPx-GI antibody
    see all

Anti-Glutathione Peroxidase 2 antibody 图像

  • Ab64322 staining human normal bladder tissue. Staining is localized to cytoplasm.
    Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Glutathione Peroxidase 2 antibody (ab64322)参考文献

ab64322 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. This product does not cross react with other GPX family. Regarding the sequence, I am afraid that the immunogen sequence is confidential and can not be released.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"