使用敲除细胞株进行验证RabMAb

Anti-Glutathione Peroxidase 1抗体[EPR3312] (ab108427)

概述

  • 产品名称Anti-Glutathione Peroxidase 1抗体[EPR3312]
    参阅全部 Glutathione Peroxidase 1 一抗
  • 描述
    兔单克隆抗体[EPR3312] to Glutathione Peroxidase 1
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Marmoset (common)
  • 免疫原

    Synthetic peptide. within Human Glutathione Peroxidase 1 aa 150 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: P07203
    (Peptide available as ab156307)

  • 阳性对照
    • Human fetal liver, 293T, THP1, and HepG2 cell lysates; Human kidney tissue; 293T cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. 

    Alternative versions available:

    Anti-Glutathione Peroxidase 1 antibody (Alexa Fluor® 488) [EPR3312] (ab197527)

    Anti-Glutathione Peroxidase 1 antibody (Alexa Fluor® 647) [EPR3312] (ab197033)
    Anti-Glutathione Peroxidase 1 antibody (HRP) [EPR3312] (ab197034)

性能

应用

Our Abpromise guarantee covers the use of ab108427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).Can be blocked with Glutathione Peroxidase 1 peptide (ab156307).
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    Anti-Glutathione Peroxidase 1 antibody [EPR3312] 图像



    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
      Lane 3: THP1 cell lysate (20 µg)
      Lane 4: HL60 cell lysate (20 µg)
      Lanes 1 and 2: Merged signal (red and green). Green - ab108427 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108427 was shown to recognize Glutathione Peroxidase 1 when Glutathione Peroxidase 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108427 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + THP-1 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size : 22 kDa
      Observed band size : 22 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/5000 dilution (purified) + Human fetal liver at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size : 22 kDa
      Observed band size : 22 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • ab108427 (purified) at 1/40 immunoprecipitating glutathione peroxidase 1 in 10 μg THP-1 cell lysate (Lanes 1 and 2, observed at 22 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : 293T cell lysate
      Lane 3 : THP1 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 22 kDa

    Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427)参考文献

    This product has been referenced in:
    • Giacci MK  et al. Differential effects of 670 and 830 nm red near infrared irradiation therapy: a comparative study of optic nerve injury, retinal degeneration, traumatic brain and spinal cord injury. PLoS One 9:e104565 (2014). IHC-Fr ; Rat . Read more (PubMed: 25105800) »
    • Koretsi V  et al. Expression of glutathione peroxidase 1 in the spheno-occipital synchondrosis and its role in ROS-induced apoptosis. Eur J Orthod N/A:N/A (2014). Read more (PubMed: 25312980) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Cell lysate - whole cell (Gonad)
    Gel Running Conditions Reduced Denaturing (12.5%)
    Loading amount 75 µg
    Specification Gonad
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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    提交于 Nov 11 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Ovary)
    Antigen retrieval step Enzymatic
    Permeabilization No
    Specification Ovary
    Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    提交于 Apr 14 2015

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
    Sample Marmoset (common) Tissue lysate - whole (brain)
    Specification brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    提交于 Apr 16 2014

    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing
    Sample Rat Tissue lysate - whole (aorta)
    Specification aorta
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Verified customer

    提交于 Oct 22 2013

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