使用敲除细胞株进行验证
重组
RabMAb

Anti-Glutathione Peroxidase 1抗体[EPR3311] (ab108429)

概述

  • 产品名称Anti-Glutathione Peroxidase 1抗体[EPR3311]
    参阅全部 Glutathione Peroxidase 1 一抗
  • 描述
    兔单克隆抗体[EPR3311] to Glutathione Peroxidase 1
  • 经测试应用适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide. within Human Glutathione Peroxidase 1 aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P07203

  • 阳性对照
    • Human fetal liver, SH SY5Y, and THP1 cell lysates; Human breast carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.

靶标

Anti-Glutathione Peroxidase 1 antibody [EPR3311] 图像



  • Predicted band size : 22 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
    Lane 3: THP1 cell lysate (20 µg)
    Lane 4: HL60 cell lysate (20 µg)
    Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108429 was shown to specifically react with Glutathione Peroxidase 1 when Glutathione Peroxidase 1 knockout samples were used. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry analysis of THP-1 (human acute monocytic leukemia ) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1:250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 22 kDa
  • ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)参考文献

This product has been referenced in:
  • Baqader NO  et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res 13:4398-423 (2014). WB ; Human . Read more (PubMed: 25133973) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab108429.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"