重组Anti-Glutathione Peroxidase 1抗体[EPR3311] (ab108429)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Glutathione Peroxidase 1抗体[EPR3311]
参阅全部 Glutathione Peroxidase 1 一抗 -
描述
兔单克隆抗体[EPR3311] to Glutathione Peroxidase 1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal liver, SH SY5Y, and THP1 cell lysates; Human breast carcinoma tissue. WB: HEK-293T cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3311 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108429于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
靶标
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功能
Protects the hemoglobin in erythrocytes from oxidative breakdown. -
序列相似性
Belongs to the glutathione peroxidase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 2876 Human
- Entrez Gene: 24404 Rat
- Omim: 138320 Human
- SwissProt: P07203 Human
- SwissProt: P04041 Rat
- Unigene: 76686 Human
- Unigene: 11323 Rat
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别名
- AL033363 antibody
- Cellular glutathione peroxidase antibody
- Glutathione peroxidase 1 antibody
see all
图片
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All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : GPX1 knockout HEK-293T cell lysate
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDaFalse colour image of Western blot: Anti-Glutathione Peroxidase 1 antibody [EPR3311] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108429 was shown to bind specifically to Glutathione Peroxidase 1. A band was observed at 22 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in GPX1 knockout cell line ab266650 (knockout cell lysate ab256932). To generate this image, wild-type and GPX1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
Lane 3: THP1 cell lysate (20 µg)
Lane 4: HL60 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108429 was shown to specifically react with Glutathione Peroxidase 1 in wild-type HAP1 cells. No band was observed when Glutathione Peroxidase 1 knockout samples were examined. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
Intracellular Flow Cytometry analysis of THP-1 (human acute monocytic leukemia) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa -
ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab108429 被引用在 11 文献中.
- Gallego-Selles A et al. Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation. Redox Biol 55:102398 (2022). PubMed: 35841628
- Sonet J et al. Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines. Int J Mol Sci 22:N/A (2021). PubMed: 34298926
- Vindry C et al. A Versatile Strategy to Reduce UGA-Selenocysteine Recoding Efficiency of the Ribosome Using CRISPR-Cas9-Viral-Like-Particles Targeting Selenocysteine-tRNA[Ser]Sec Gene. Cells 8:N/A (2019). PubMed: 31212706
- Sonet J et al. Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines. Nutrients 11:N/A (2019). PubMed: 31277500
- Jin L et al. The PLAG1-GDH1 Axis Promotes Anoikis Resistance and Tumor Metastasis through CamKK2-AMPK Signaling in LKB1-Deficient Lung Cancer. Mol Cell 69:87-99.e7 (2018). PubMed: 29249655