Anti-Glutathione Peroxidase 1抗体(ab59546)


  • 产品名称Anti-Glutathione Peroxidase 1抗体
    参阅全部 Glutathione Peroxidase 1 一抗
  • 描述
    兔多克隆抗体to Glutathione Peroxidase 1
  • 特异性ab59546 recognises human and rat cellular selenium dependant glutathione peroxidase
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Pig, Chimpanzee, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Glutathione Peroxidase 1 aa 175-194.


  • 阳性对照
    • Human brain tissue cytoplasmic lysate Human or rat glial cells Human or rat neurons Stains human and rat glial cells very strongly and shows lesser staining in neurons.
  • 常规说明Store working volume at +4°C.



Our Abpromise guarantee covers the use of ab59546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/20 - 1/100.
WB 1/1000 - 1/4000. Detects a band of approximately 22 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


Anti-Glutathione Peroxidase 1 antibody 图像

  • ab59546, at a 1/100 dilution, staining rat Cellular Glutathione Peroxidase in brain neurons using Immunohistochemistry. Secondary antibody donkey anti-rabbit conjugated to Cy3 at a 1/100 dilution.
  • Anti-Glutathione Peroxidase 1 antibody (ab59546) at 1/2000 dilution + Liver (Mouse) Tissue Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab59546 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59546, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab59546 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59546, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glutathione Peroxidase 1 antibody (ab59546)参考文献

This product has been referenced in:
  • Bhatt NM  et al. Restoring redox balance enhances contractility in heart trabeculae from type 2 diabetic rats exposed to high glucose. Am J Physiol Heart Circ Physiol 308:H291-302 (2015). WB ; Rat . Read more (PubMed: 25485897) »
  • Quintana-Cabrera R  et al. G-glutamylcysteine detoxifies reactive oxygen species by acting as glutathione peroxidase-1 cofactor. Nat Commun 3:718 (2012). WB ; Human . Read more (PubMed: 22395609) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions Reduced Denaturing (4-15% Gradient)
Loading amount 20 µg
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Julie Kim

Verified customer

提交于 May 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (LIVER)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification LIVER
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 14 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Uterus)
Specification Uterus
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Microwaves, 2x5 min Citrate buffer pH 6
Permeabilization No
Blocking step Polyvalent blocking serum (Ultra V Block® , Thermo Fisher Scientific, LabVision Corporation) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C

Ms. Rita Payan Carreira

Verified customer

提交于 Sep 07 2011

Application Western blot
Sample Pig Tissue lysate - whole (Coronary arteries and arterioles)
Loading amount 20 µg
Specification Coronary arteries and arterioles
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Dr. Wei Xie

Verified customer

提交于 Jul 28 2009