Anti-Glutathione Peroxidase 1抗体(ab59546)

概述

  • 产品名称
    Anti-Glutathione Peroxidase 1抗体
    参阅全部 Glutathione Peroxidase 1 一抗
  • 描述
    兔多克隆抗体to Glutathione Peroxidase 1
  • 特异性
    ab59546 recognises human and rat cellular selenium dependant glutathione peroxidase
  • 经测试应用
    适用于: ICC/IF, WB, ELISA, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Pig, Chimpanzee, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Glutathione Peroxidase 1 aa 175-194.
    Sequence:

    RRYSRRFQTIDIEPDIEALL

  • 阳性对照
    • Human brain tissue cytoplasmic lysate Human or rat glial cells Human or rat neurons Stains human and rat glial cells very strongly and shows lesser staining in neurons.
  • 常规说明
    Store working volume at +4°C.

性能

应用

Our Abpromise guarantee covers the use of ab59546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/20 - 1/100.
WB 1/1000 - 1/4000. Detects a band of approximately 22 kDa.
ELISA 1/1000 - 1/4000.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

图片

  • ab59546, at a 1/100 dilution, staining rat Cellular Glutathione Peroxidase in brain neurons using Immunohistochemistry. Secondary antibody donkey anti-rabbit conjugated to Cy3 at a 1/100 dilution.
  • Anti-Glutathione Peroxidase 1 antibody (ab59546) at 1/2000 dilution + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab59546 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59546, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab59546 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59546, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • González-Torres L  et al. Glucomannan- and glucomannan plus spirulina-enriched pork affect liver fatty acid profile, LDL receptor expression and antioxidant status in Zucker fa/fa rats fed atherogenic diets. Food Nutr Res 61:1264710 (2017). WB . Read more (PubMed: 28325998) »
  • Santos-López JA  et al. Effects of Silicon vs. Hydroxytyrosol-Enriched Restructured Pork on Liver Oxidation Status of Aged Rats Fed High-Saturated/High-Cholesterol Diets. PLoS One 11:e0147469 (2016). Read more (PubMed: 26807847) »

See all 5 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% Gradient)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Julie Kim

Verified customer

提交于 May 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
LIVER
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Uterus)
Specification
Uterus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Microwaves, 2x5 min Citrate buffer pH 6
Permeabilization
No
Blocking step
Polyvalent blocking serum (Ultra V Block® , Thermo Fisher Scientific, LabVision Corporation) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Ms. Rita Payan Carreira

Verified customer

提交于 Sep 07 2011

Application
Western blot
Sample
Pig Tissue lysate - whole (Coronary arteries and arterioles)
Loading amount
20 µg
Specification
Coronary arteries and arterioles
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. Wei Xie

Verified customer

提交于 Jul 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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