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Our Abpromise guarantee covers the use of ab50427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 3 - 5 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|ELISA||Use at an assay dependent concentration. Antibody detection limit dilution 1:32,000.|
ab50427 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney following microwave antigen retrieval with citrate buffer pH 3 and CY3-staining shows staining of cytoplasm in PCT epithelial cells.
ab50437 at 3µg/ml staining Glutathione Perxidase 1 in human cerebral cortex by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used for staining.