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Our Abpromise guarantee covers the use of ab19534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19833109|
|WB||1/1000. Use under non reducing condition. We recommend blocking with 5% milk (not BSA). While glutathione itself is too small to detect in WB, this antibody will detect all glutathionylated proteins. You may observe multiple bands at variable molecular weights depending on what proteins in your samples are glutathionylated.|
|IHC-Fr||1/100 - 1/200.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"