Anti-Glutamyl Prolyl tRNA synthetase抗体(ab31531)

概述

  • 产品名称Anti-Glutamyl Prolyl tRNA synthetase抗体
    参阅全部 Glutamyl Prolyl tRNA synthetase 一抗
  • 描述
    兔多克隆抗体to Glutamyl Prolyl tRNA synthetase
  • 特异性This antibody reacts specifically with EPRS protein.
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Pig
  • 免疫原

    V573-K889 native human recombinant EPRS.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab31531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21460219
WB 1/20000. Predicted molecular weight: 163 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
  • 序列相似性In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
    In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 3 WHEP-TRS domains.
  • 结构域The WHEP-TRS domain is involved in RNA binding.
  • 翻译后修饰Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bifunctional aminoacyl tRNA synthetase antibody
    • Bifunctional aminoacyl-tRNA synthetase antibody
    • Bifunctional glutamate/proline tRNA ligase antibody
    • Cell proliferation-inducing gene 32 protein antibody
    • DKFZp313B047 antibody
    • EARS antibody
    • Eprs antibody
    • GLNS antibody
    • Glu pro tRNA synthetase antibody
    • GLUPRORS antibody
    • GluRS antibody
    • Glutamate tRNA ligase antibody
    • Glutamatyl prolyl tRNA synthetase antibody
    • Glutaminyl tRNA synthetase antibody
    • Glutamyl prolyl tRNA synthetase antibody
    • Glutamyl tRNA synthetase antibody
    • Glutamyl-tRNA synthetase antibody
    • PARS antibody
    • PIG 32 antibody
    • PIG32 antibody
    • Proliferation inducing gene 32 protein antibody
    • Proliferation inducing protein 32 antibody
    • Proline tRNA ligase antibody
    • Proline--tRNA ligase antibody
    • Prolyl tRNA synthetase antibody
    • Prolyl-tRNA synthetase antibody
    • QARS antibody
    • QPRS antibody
    • SYEP_HUMAN antibody
    see all

Anti-Glutamyl Prolyl tRNA synthetase antibody 图像

  • Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531) at 1/10000 dilution + HEK293 cell line at 30 µg

    Predicted band size : 163 kDa
    Observed band size : 163 kDa
  • Ab31531 staining Glutamyl Prolyl tRNA synthetase in Hela cell line by Immunofluorescence.
  • Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531) at 1/20000 dilution + whole cell lysate prepared from HEK-293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 163 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab31531 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31531, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab31531 staining Glutamyl Prolyl tRNA synthetase in the Human Liver cell line Huh7 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.5% and blocked with 10% Serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 10% Serum) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531)参考文献

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Kim K  et al. Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One 8:e81734 (2013). WB, IP ; Human . Read more (PubMed: 24312579) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Liver)
Total protein in input 1e+007 cells
Specification Liver
Immuno-precipitation step Other - MagnaChip Protein A+G
Username

Abcam user community

Verified customer

提交于 Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Huh7 (Liver))
Loading amount 1000 cells
Specification Huh7 (Liver)
Gel Running Conditions Reduced Denaturing (4-12 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Silvia Marquez Jurado

Verified customer

提交于 Aug 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Huh7 (Liver))
Specification Huh7 (Liver)
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X100 0,5%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (testis)
Specification testis
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X100 0,5%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell lysate)
Loading amount 10 µg
Specification HeLa cell lysate
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 25 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

提交于 Mar 26 2010

Thank you for your email and for the database information for this protein, we'll add the information to the datasheet. This antibody was tested in Western blotting using a HEK293 cell lysate and in IF using Hela cells. We have not tested the antibody ...

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