Anti-Glutamine Synthetase抗体(ab93439)

概述

  • 产品名称
    Anti-Glutamine Synthetase抗体
    参阅全部 Glutamine Synthetase 一抗
  • 描述
    兔多克隆抗体to Glutamine Synthetase
  • 经测试应用
    适用于: WB, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Zebrafish
    预测可用于: Chicken, Cow, Dog, Human, Pig, Cynomolgus monkey, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Glutamine Synthetase.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cerebellum; Rat Cerebellum; Mouse Kidney; Rat Kidney; Mouse Liver.

性能

应用

Our Abpromise guarantee covers the use of ab93439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
IHC-Fr 1/100.
IHC-FoFr 1/1000.

靶标

  • 功能
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • 疾病相关
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • 序列相似性
    Belongs to the glutamine synthetase family.
  • 发展阶段
    Expressed during early fetal stages.
  • 细胞定位
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

图片

  • ab93439 staining Glutamine Synthetase in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab93439 at 1/100 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Glutamine Synthetase expression can be observed in Muller cells (in purple).

    See Abreview

  • All lanes : Anti-Glutamine Synthetase antibody (ab93439) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Cerebellum (Mouse) Tissue Lysate
    Lane 4 : Cerebellum (Rat) Tissue Lysate
    Lane 5 : Kidney (Mouse) Tissue Lysate
    Lane 6 : Kidney (Rat) Tissue Lysate
    Lane 7 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • IHC-FoFr image of Glutamine Synthetase staining on rat brain sections using ab93439 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

文献

ab93439 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - PBStx 3x5mn RT
Specification
brain
Blocking step
Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Fixative
Paraformaldehyde
Username

Yacine Tensaouti

Verified customer

提交于 Aug 02 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller cells)
Specification
Retina, Muller cells
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Ryan Macdonald

Verified customer

提交于 Jul 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册