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Our Abpromise guarantee covers the use of ab73593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Mouse Glutamine Synthetase peptide (ab73592).|
Immunohistochemistical detection of Glutamine Synthetase on Formaldehyde-fixed paraffin-embedded rat brain sections using Glutamine Synthetase antibody (ab73593). Antigen retrieval step: Heat mediated - Buffer/Enzyme Used: Citric acid pH6 Permeabilization. Blocking: 1% BSA for 10 mins at room temperature. Primary Antibody Dilution 1/1000. Incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG Conjugated to biotin (1/200). In the submitted image of rat cerebellum, two very good examples are observed of the bulbous endfeet of the primary Bergmann glia primary processes connecting with the inner Pial membrane.
ICC/IF image of ab73593 stained MEF cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73593, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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