Anti-Glutamine Synthetase抗体(ab73593)

概述

  • 产品名称
    Anti-Glutamine Synthetase抗体
    参阅全部 Glutamine Synthetase 一抗
  • 描述
    兔多克隆抗体to Glutamine Synthetase
  • 经测试应用
    适用于: IHC-FoFr, IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human, Common marmoset
    预测可用于: Hamster, Cow, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: P15105
    (Peptide available as ab73592)

  • 阳性对照
    • WB: Mouse kidney, mouse liver, rat liver, human brain, mouse brain, rat lung and mouse pancreas (data not shown) tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab73593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/3000.
IHC-P 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Mouse Glutamine Synthetase peptide (ab73592).

靶标

  • 功能
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • 疾病相关
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • 序列相似性
    Belongs to the glutamine synthetase family.
  • 发展阶段
    Expressed during early fetal stages.
  • 细胞定位
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

Anti-Glutamine Synthetase antibody 图像

  • Immunohistochemistical detection of Glutamine Synthetase on Formaldehyde-fixed paraffin-embedded rat brain sections using Glutamine Synthetase antibody (ab73593).  Antigen retrieval step: Heat mediated - Buffer/Enzyme Used: Citric acid pH6 Permeabilization. Blocking: 1% BSA for 10 mins at room temperature. Primary Antibody Dilution 1/1000. Incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG Conjugated to biotin (1/200). In the submitted image of rat cerebellum, two very good examples are observed of the bulbous endfeet of the primary Bergmann glia primary processes connecting with the inner Pial membrane.

  • All lanes : Anti-Glutamine Synthetase antibody (ab73593) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Brain (Mouse) Tissue Lysate
    Lane 6 : Lung (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 30 seconds
  • ICC/IF image of ab73593 stained MEF cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73593, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC-FoFr image of Glutamine Synthetase (ab73593) staining on Rat hippocampus sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa


    Exposure time : 2 minutes

Anti-Glutamine Synthetase antibody (ab73593)参考文献

This product has been referenced in:
  • Andersen JV  et al. Impaired Hippocampal Glutamate and Glutamine Metabolism in the db/db Mouse Model of Type 2 Diabetes Mellitus. Neural Plast 2017:2107084 (2017). WB ; Mouse . Read more (PubMed: 28695014) »
  • Sheppard S  et al. The immunoreceptor NKG2D promotes tumour growth in a model of hepatocellular carcinoma. Nat Commun 8:13930 (2017). IHC . Read more (PubMed: 28128200) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Rat Cell lysate - whole cell (neuronal cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
neuronal cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 27 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (B lymphoblast)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
B lymphoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2017

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Muller Cell)
Specification
Retina, Muller Cell
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Ryan Macdonald

Verified customer

提交于 May 28 2013

Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2013

Thank you for your inquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storag...

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Thank you for contacting us in regards to anti-Glutamine Synthetase antibody (ab73593).

I now have confirmation form the lab of how the Western blot presented on the datasheet for this antibody was performed. We used 10 ug of the different ti...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain, spinal cord)
Specification
Brain, spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

提交于 Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain - Hippocampus)
Specification
Brain - Hippocampus
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (P5 cerebellum)
Specification
P5 cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jul 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 26 2010

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