Anti-Glutamine Synthetase抗体(ab64613)

概述

  • 产品名称Anti-Glutamine Synthetase抗体
    参阅全部 Glutamine Synthetase 一抗
  • 描述
    小鼠单克隆抗体to Glutamine Synthetase
  • 经测试应用适用于: ELISA, WB, IHC-FoFr, IHC-Fr, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Drosophila melanogaster, Zebrafish, Apteronotus leptorhynchus
    预测可用于: Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Cynomolgus Monkey, Opossum, Chinese Hamster
  • 免疫原

    Recombinant fragment with tag: IEKLSKRHQY HIRAYDPKGG LDNARRLTGF HETSNINDFS AGVANRSASI RIPRTVGQEK KGYFEDRRPS ANCDPFSVTE ALIRTCLLNE TGDEPFQYKN , corresponding to amino acids 274-374 of Human Glutamine Synthetase

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab64613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. In sELISA the detection limit for recombinant tagged Glutamine Synthetase is approximately 0.03ng/ml as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
IHC-FoFr Use at an assay dependent concentration. PubMed: 23374330
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-Glutamine Synthetase antibody 图像

  • All lanes : Anti-Glutamine Synthetase antibody (ab64613) at 0.5 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish liver homogenate at 20 µg
    Lane 4 : Zebrafish skeletal muscle homogenate at 20 µg
    Lane 5 : JURKAT (Human T cell lymphoblast-like cell line) whole cell lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 4 minutes
  • ICC/IF image of ab64613 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64613, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab64613 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64613, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing Jurkat cells stained with ab64613 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab64613, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-Glutamine Synthetase antibody (ab64613) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 42 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-Glutamine Synthetase antibody (ab64613)参考文献

This product has been referenced in:
  • Ballantyne LL  et al. Liver-specific knockout of arginase-1 leads to a profound phenotype similar to inducible whole body arginase-1 deficiency. Mol Genet Metab Rep 9:54-60 (2016). IHC-P, IF ; Mouse . Read more (PubMed: 27761413) »
  • Yu D  et al. Kidney-type glutaminase (GLS1) is a biomarker for pathologic diagnosis and prognosis of hepatocellular carcinoma. Oncotarget 6:7619-31 (2015). IHC ; Human . Read more (PubMed: 25844758) »

See all 8 Publications for this product

Product Wall

Gracias por contactarnos.

He llevado a cabo una búsqueda con los requisitos indicados, y me temo que no hay en el catálogo ningún anticuerpo que cumpla con todos ellos.

Sin embargo, existen otras alternativas co...

Read More
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, Muller glia cells)
Specification Retina, Muller glia cells
Permeabilization Yes - 0.1% TritonX
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

提交于 May 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 03 2012

Gracias por tu respuesta.

En ese caso, por favor corrígeme si me equivoco, el cliente estaría interesado en usar secciones fijadas por perfusión y posteriormente congeladas. Seria pues la aplicación de immunohistoq...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

提交于 Nov 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Drosophila S2 cell)
Loading amount 20 µg
Specification Drosophila S2 cell
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Myoung Sup Shim

Verified customer

提交于 Apr 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"