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Synthetic peptide corresponding to Human Glutaminase aa 516-545 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab93434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/1000. Predicted molecular weight: 65 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
ICC/IF image of ab93434 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93434, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Glutaminase (green) with ab93434. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counter stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling Glutaminase with ab93434. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody.
Flow cytometry analysis of HepG2 cells labelling Glutaminase (green) with ab93434 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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