Anti-Glutamate Transporter 1抗体(ab106289)

Abreviews (2)Q&A (1)Specific References (3)

概述

  • 产品名称
    Anti-Glutamate Transporter 1抗体
  • 描述
    兔多克隆抗体to Glutamate Transporter 1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    A 16 residue synthetic peptide derived from the C terminal domain of Rat glutamate transporter 1.

  • 阳性对照
    • WB: Extracts from rat and mouse brains.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab106289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/200 - 1/5000. Predicted molecular weight: 62 kDa.
Flow Cyt 1/10 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

靶标

图片

  • Western blot - Glutamate Transporter 1 antibody (ab106289)
    Western blot - Glutamate Transporter 1 antibody (ab106289)
    All lanes : Anti-Glutamate Transporter 1 antibody (ab106289) at 1/1000 dilution

    Lane 1 : Protein extract from rat brain
    Lane 2 : Protein extract from mouse brain

    Predicted band size: 62 kDa



    Lane 3 shows inhibition of the immunoreactivity of ab106289 with blocking peptide.
    Glutamate Transporter 1 protein is detected at 35 kDa and 70 kDa as a dimer.

文献

This product has been referenced in:
  • Lakatos A  et al. Integrated analysis of genetic, behavioral, and biochemical data implicates neural stem cell-induced changes in immunity, neurotransmission and mitochondrial function in Dementia with Lewy Body mice. Acta Neuropathol Commun 5:21 (2017). Read more (PubMed: 28283027) »
  • Dai J  et al. Alterations in AQP4 expression and polarization in the course of motor neuron degeneration in SOD1G93A mice. Mol Med Rep 16:1739-1746 (2017). Read more (PubMed: 28627708) »

See all 3 Publications for this product

发表研究结果有使用 ab106289？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-Glutamate Transporter 1 antibody

 Below Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (IPSC derived astrocyte)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
IPSC derived astrocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
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Verified customer

提交于 Mar 02 2017

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glutamate Transporter 1 antibody

 Average
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Apr 23 2013

send testing discount for testing ab106289 in human in ihc


Je suis ravie que vous souhaitiez participer a notre offre et tester ab106289 sur coupes de tissu humain. Le code de réduction correspondant est xx . Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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