Anti-Glutamate receptor ionotropic, NMDA 2D抗体(ab35448)

概述

  • 产品名称Anti-Glutamate receptor ionotropic, NMDA 2D抗体
    参阅全部 Glutamate receptor ionotropic, NMDA 2D 一抗
  • 描述
    兔多克隆抗体to Glutamate receptor ionotropic, NMDA 2D
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to Human Glutamate receptor ionotropic, NMDA 2D aa 1300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O15399
    (Peptide available as ab35447)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Brain Tissue Lysate (day 0 and day 7) and Rat Brain Tissue Lysate (day 0 and day 7)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab35448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).Can be blocked with Rat Glutamate receptor ionotropic or NMDA 2D peptide (ab35447).

靶标

  • 功能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily.
  • 细胞定位Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EB11 antibody
    • estrogen receptor binding CpG island antibody
    • GluN2D antibody
    • Glutamate [NMDA] receptor subunit epsilon 4 antibody
    • Glutamate [NMDA] receptor subunit epsilon-4 antibody
    • Glutamate receptor ionotropic antibody
    • glutamate receptor ionotropic NMDA 2D antibody
    • Glutamate receptor, ionotropic, N-methyl D-aspartate 2D antibody
    • Grin2d antibody
    • N methyl D aspartate receptor subtype 2D antibody
    • N methyl d aspartate receptor subunit 2D antibody
    • N-methyl D-aspartate receptor subtype 2D antibody
    • NMDA 2D antibody
    • NMDAR2D antibody
    • NMDE4 antibody
    • NMDE4_HUMAN antibody
    • NR2D antibody
    see all

Anti-Glutamate receptor ionotropic, NMDA 2D antibody 图像

  • All lanes : Anti-Glutamate receptor ionotropic, NMDA 2D antibody (ab35448) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old
    Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old
    Lane 4 : Brain (Rat) Tissue Lysate, 7 days old

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 143 kDa
    Observed band size : 143 kDa
    Additional bands at : 125 kDa,45 kDa,60 kDa,~200 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.

Anti-Glutamate receptor ionotropic, NMDA 2D antibody (ab35448)参考文献

This product has been referenced in:
  • Zhou X  et al. Reduced NMDAR1 expression in the Sp4 hypomorphic mouse may contribute to endophenotypes of human psychiatric disorders. Hum Mol Genet 19:3797-805 (2010). WB ; Mouse . Read more (PubMed: 20634195) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (e12 PNS and adult cerebellum)
Specification e12 PNS and adult cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid ph6
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jul 19 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"