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RabMAb

Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845)抗体[EPR2148] (ab76321)

概述

  • 产品名称
    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845)抗体[EPR2148]
    参阅全部 Glutamate Receptor 1 (AMPA subtype) 一抗
  • 描述
    兔单克隆抗体[EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
  • 特异性
    ab76321 only detects Glutamate Receptor 1 (AMPA subtype) phosphorylated on Serine 845.
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glutamate Receptor 1 (AMPA subtype) (phospho S845).

  • 阳性对照
    • WB: Human fetal, rat and mouse brain tissue lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab76321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 102 kDa.

For unpurified use at 1/1000 - 1/10000.

  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能
      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • 组织特异性
      Widely expressed in brain.
    • 序列相似性
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
    • 翻译后修饰
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • 细胞定位
      Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GLUR 1 antibody
      • GLUR A antibody
      • AMPA 1 antibody
      • AMPA selective glutamate receptor 1 antibody
      • AMPA-selective glutamate receptor 1 antibody
      • GluA1 antibody
      • GLUH1 antibody
      • GluR K1 antibody
      • GluR-1 antibody
      • GluR-A antibody
      • GluR-K1 antibody
      • GLUR1 antibody
      • GLURA antibody
      • GluRK1 antibody
      • Glutamate receptor 1 antibody
      • Glutamate receptor ionotropic AMPA 1 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor, ionotropic, AMPA 1 antibody
      • Gria1 antibody
      • GRIA1_HUMAN antibody
      • HBGR1 antibody
      • MGC133252 antibody
      • OTTHUMP00000160643 antibody
      • OTTHUMP00000165781 antibody
      • OTTHUMP00000224241 antibody
      • OTTHUMP00000224242 antibody
      • OTTHUMP00000224243 antibody
      see all

    图片

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/1000 dilution

      Lane 1 : Mouse hippocampus whole cell lysates
      Lane 2 : Mouse hippocampus whole cell lysates. The membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 102 kDa
      Observed band size : 106 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocked in 5% NFDM/TBST

    • Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) labelling Glutamate Receptor 1 (AMPA subtype) (phospho S845) with ab76321 at 1/1000 in 5% NFDM/TBST. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Exposed for 3 minutes.

      Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS845) phospho peptide  

      Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/1000 dilution

      Lane 1 : Human cerebellum whole cell lysates
      Lane 2 : Human cerebellum whole cell lysates. The membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 102 kDa
      Observed band size : 106 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocked with 5% NFDM/TBST

    • Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1000 cells (purified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate, untreated
      Lane 2 : Mouse brain tissue lysate, treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)

      Lane 1 : Rat brain tissue lysate, untreated
      Lane 2 : Rat brain tissue lysate, treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (unpurified)

      Lane 1 : Rat brain tissue lysate, untreated
      Lane 2 : Rat brain tissue lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 106 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    This product has been referenced in:
    • Kang WB  et al. Analgesic effects of adenylyl cyclase inhibitor NB001 on bone cancer pain in a mouse model. Mol Pain 12:N/A (2016). WB . Read more (PubMed: 27612915) »
    • Hao JR  et al. L-Stepholidine rescues memory deficit and synaptic plasticity in models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway. Cell Death Dis 6:e1965 (2015). WB . Read more (PubMed: 26539912) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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