RabMAb

Anti-Glutamate Receptor 1 (AMPA subtype)抗体[EPR5479] (ab109450)

概述

  • 产品名称Anti-Glutamate Receptor 1 (AMPA subtype)抗体[EPR5479]
    参阅全部 Glutamate Receptor 1 (AMPA subtype) 一抗
  • 描述
    兔单克隆抗体[EPR5479] to Glutamate Receptor 1 (AMPA subtype)
  • 经测试应用适用于: IHC-Fr, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glutamate Receptor 1 (AMPA subtype) aa 850 to the C-terminus (C terminal).
    Database link: P42261

  • 阳性对照
    • WB: Human, Mouse and Rat brain lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
WB 1/2000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).

For unpurified use at 1/10000 - 1/50000.

IP 1/10 - 1/100.

靶标

  • 功能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • 组织特异性Widely expressed in brain.
  • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
  • 翻译后修饰Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GLUR 1 antibody
    • GLUR A antibody
    • AMPA 1 antibody
    • AMPA selective glutamate receptor 1 antibody
    • AMPA-selective glutamate receptor 1 antibody
    • GluA1 antibody
    • GLUH1 antibody
    • GluR K1 antibody
    • GluR-1 antibody
    • GluR-A antibody
    • GluR-K1 antibody
    • GLUR1 antibody
    • GLURA antibody
    • GluRK1 antibody
    • Glutamate receptor 1 antibody
    • Glutamate receptor ionotropic AMPA 1 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor, ionotropic, AMPA 1 antibody
    • Gria1 antibody
    • GRIA1_HUMAN antibody
    • HBGR1 antibody
    • MGC133252 antibody
    • OTTHUMP00000160643 antibody
    • OTTHUMP00000165781 antibody
    • OTTHUMP00000224241 antibody
    • OTTHUMP00000224242 antibody
    • OTTHUMP00000224243 antibody
    see all

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] 图像

  • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/5000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 102 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution (purified) + Human brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 102 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution (unpurified)

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 102 kDa
    Observed band size : 106 kDa (why is the actual band size different from the predicted?)
  • ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/1,500) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)参考文献

This product has been referenced in:
  • Chen C  et al. Epigenetic modification of PKM? rescues aging-related cognitive impairment. Sci Rep 6:22096 (2016). WB ; Rat . Read more (PubMed: 26926225) »
  • Gascon E  et al. Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nat Med 20:1444-51 (2014). WB ; Mouse . Read more (PubMed: 25401692) »

See all 3 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Mouse Tissue lysate - other (Hippocampus)
Specification Hippocampus
Total protein in input 5 µg
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提交于 Feb 11 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Hippocampus)
Specification Hippocampus
Permeabilization No
Fixative Formaldehyde
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Verified customer

提交于 Feb 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"