Anti-Glutamate Receptor 1 (AMPA subtype)抗体[EPR5479] (ab109450)
- 数据表
- 具体的参考文献 (9)
- 实验方案
概述
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产品名称Anti-Glutamate Receptor 1 (AMPA subtype)抗体[EPR5479]
参阅全部 Glutamate Receptor 1 (AMPA subtype) 一抗 -
描述兔单克隆抗体[EPR5479] to Glutamate Receptor 1 (AMPA subtype)
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宿主Rabbit
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经测试应用适用于: IHC-Fr, WB, IPmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glutamate Receptor 1 (AMPA subtype) aa 850 to the C-terminus (C terminal).
Database link: P42261 -
阳性对照
- WB: Human, Mouse and Rat brain lysates.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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解离常数（KD）KD = 1.94 x 10 -11 M Learn more about KD
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EPR5479
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab109450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | 1/100. | |
| WB | 1/2000. Detects a band of approximately 106 kDa (predicted molecular weight: 102 kDa).Can be blocked with Glutamate Receptor 1 (AMPA subtype) peptide (ab219618). For unpurified use at 1/10000 - 1/50000. |
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| IP | 1/10 - 1/100. |
靶标
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功能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
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组织特异性Widely expressed in brain.
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序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
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翻译后修饰Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
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细胞定位Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
- Information by UniProt
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数据库链接
- Entrez Gene: 2890 Human
- Entrez Gene: 14799 Mouse
- Entrez Gene: 50592 Rat
- Omim: 138248 Human
- SwissProt: P42261 Human
- SwissProt: P23818 Mouse
- SwissProt: P19490 Rat
- Unigene: 519693 Human
see all -
别名
- GLUR 1 antibody
- GLUR A antibody
- AMPA 1 antibody
see all
图片
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![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](http://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-5-ab109450WB2.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/5000 dilution (purified)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 102 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](http://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-4-ab109450WB1.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/2000 dilution (purified) + Human brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 102 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](http://www.abcam.cn/ps/datasheet/images/109/ab109450/Glutamate-Receptor-1-AMPA-subtype-Primary-antibodies-ab109450-1.jpg)
Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450) at 1/1000 dilution (unpurified)
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa
Observed band size: 106 kDa (why is the actual band size different from the predicted?) -
![Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](http://www.abcam.cn/ps/products/109/ab109450/Images/ab109450-3-ab109450IP1.jpg)
Immunoprecipitation - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)ab109450 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in Mouse Brain whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/1,500) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Other - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)](http://www.abcam.cn/ps/datasheet/images/109/ab109450/Glutamate-Receptor-1-AMPA-subtype-Primary-antibodies-ab109450-2.jpg)
Other - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (ab109450)
文献
This product has been referenced in:
- Schmitz LJM et al. The AMPA receptor-associated protein Shisa7 regulates hippocampal synaptic function and contextual memory. Elife 6:N/A (2017). Read more (PubMed: 29199957) »
- Huang X et al. Methamphetamine abuse impairs motor cortical plasticity and function. Mol Psychiatry 22:1274-1281 (2017). Read more (PubMed: 28831198) »
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