RabMAb

Anti-Glutamate Receptor 1 (AMPA subtype)抗体[E308] (ab32436)

概述

  • 产品名称Anti-Glutamate Receptor 1 (AMPA subtype)抗体[E308]
    参阅全部 Glutamate Receptor 1 (AMPA subtype) 一抗
  • 描述
    兔单克隆抗体[E308] to Glutamate Receptor 1 (AMPA subtype)
  • 特异性This antibody is specific for Glutamate Receptor 1 (AMPA subtype).
  • 经测试应用适用于: WB, IHC-P, IPmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminus of Human Glutamate Receptor 1 (AMPA subtype), within the first 100 amino acids.

  • 阳性对照
    • WB: SH-SY5Y cell lysate. IHC: human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab32436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 101 kDa).
IHC-P 1/50.
IP 1/30.
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • 组织特异性Widely expressed in brain.
    • 序列相似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
    • 翻译后修饰Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
    • Information by UniProt
    • 数据库链接
    • 别名
      • GLUR 1 antibody
      • GLUR A antibody
      • AMPA 1 antibody
      • AMPA selective glutamate receptor 1 antibody
      • AMPA-selective glutamate receptor 1 antibody
      • GluA1 antibody
      • GLUH1 antibody
      • GluR K1 antibody
      • GluR-1 antibody
      • GluR-A antibody
      • GluR-K1 antibody
      • GLUR1 antibody
      • GLURA antibody
      • GluRK1 antibody
      • Glutamate receptor 1 antibody
      • Glutamate receptor ionotropic AMPA 1 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor, ionotropic, AMPA 1 antibody
      • Gria1 antibody
      • GRIA1_HUMAN antibody
      • HBGR1 antibody
      • MGC133252 antibody
      • OTTHUMP00000160643 antibody
      • OTTHUMP00000165781 antibody
      • OTTHUMP00000224241 antibody
      • OTTHUMP00000224242 antibody
      • OTTHUMP00000224243 antibody
      see all

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] 图像

    • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution + SH-SY5Y cell lysate.

      Predicted band size : 101 kDa
      Observed band size : 106 kDa (why is the actual band size different from the predicted?)
    • Ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry.
    • Immunohistochemical analysis of Human hippocampus tissue, staining Glutamate Receptor 1 (AMPA subtype) with ab32436.

      Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Samples were blocked with 10% normal goat serum for 30 minutes before incubating with primary antibody (1/20) for 2 hours. A biotinylated anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)参考文献

    This product has been referenced in:
    • Plaza-Zabala A  et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure. Neurosci Lett 557 Pt B:101-6 (2013). WB ; Rat . Read more (PubMed: 24262606) »
    • van Vuurden DG  et al. Attenuated AMPA receptor expression allows glioblastoma cell survival in glutamate-rich environment. PLoS One 4:e5953 (2009). IHC-P ; Human . Read more (PubMed: 19536293) »

    See all 2 Publications for this product

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