Glutamate Dehydrogenase Detection试剂盒(ab102527)

概述

  • 产品名称Glutamate Dehydrogenase Detection试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Enzyme activity
  • 灵敏度
    > 0.01 mU/well
  • 产品概述

    Glutamate Dehydrogenase Detection Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (? = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to a-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to alpha-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
  • 别名
    • GDH

应用

Our Abpromise guarantee covers the use of ab102527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Glutamate Dehydrogenase Detection Kit 图像

  • GDH measured showing activity (mU) per mL of tested sample

  • GDH measured in cell lysates showing activity (mU) per 106 cells tested

  • GDH measured in various samples showing O.D.'s in time course

  • NADH Standard Curve

实验方案

Glutamate Dehydrogenase Detection Kit (ab102527)参考文献

ab102527 has not yet been referenced specifically in any publications.

Product Wall

- No special preparation is required for cell culture supernatant. Simply collect the media and use in the assay. It is worth testing different dilutions with GDH assay buffer. I would suggest using 5-50 ul as with serum samples, bringing the final vol...

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Thank you for contacting Abcam regarding ab102527.


We have not tested this kit with lactobacilli, but theoretically it should work. Further protocol optimization may be necessary for best results.


Unfortunately, we do no...

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Thank you for your patience.

Unfortunately, the species protein is propriety and I cannot give you this information. However, the lab just confirmed that this kitab102527 Glutamate Dehydrogenase DetectionKitis suitable for sheep serum.
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Thank you for contacting us.

Unfortunately, we just have the aforementioned kit you mentioned butnothing regarding the other enzymes.

As the species is not given, I assumed that the test was established using the human protein. I c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"