Anti-Glutamate抗体[2Q71] (ab17463)

概述

  • 产品名称Anti-Glutamate抗体[2Q71]
    参阅全部 Glutamate 一抗
  • 描述
    小鼠单克隆抗体[2Q71] to Glutamate
  • 特异性The crossreactivities were determined using an ELISA test by competition experiments with the following compounds: Compound Crossreactivity Glutamate-G-BSA 1 Glutamate=G=BSA 1:10 Aspartate-G-BSA 1:100,000 GABA-G-BSA 1:100,000 Glutamate 1:100,000.
  • 经测试应用适用于: IHC-FoFrmore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Glutamate-Glutaraldehyde-BSA.

性能

应用

Our Abpromise guarantee covers the use of ab17463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr
  • 应用说明IHC-FoFr: 1/2500 - 1/10000 (tested using free floating sections by the PAP technique on rat hippocampus).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Glutamate is considered the principle excitatory amino acid in the CNS. Its role in cellular metabolism is well known. Glumate reactivity is particularly observed in the granular cells of the cerebellum, the pyramidal cells of the hippocampus, the Betz cells of the motor strip, and the projections of the frontal lobe to the basal ganglia.
    • 别名
      • D glutamate antibody
      • Glutamate antibody

    Anti-Glutamate antibody [2Q71] (ab17463)参考文献

    ab17463 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Verified customer

    提交于 Apr 10 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.3% Triton X-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 05 2013

    Thank you for your enquiry. This antibody has been tested in IHC-free floating sections and has not yet been tested in IHC-frozen sections. For the free floating sections, a dilution range of 1/2500 - 1/10000 is recommended. The PAP technique (pero...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"