Anti-GLUT12抗体(ab100993)

概述

  • 产品名称Anti-GLUT12抗体
    参阅全部 GLUT12 一抗
  • 描述
    兔多克隆抗体to GLUT12
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GLUT12.

  • 阳性对照
    • This antibody gave a positive signal in both Human liver membrane and Human liver tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab100993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 67 kDa).

靶标

  • 功能Facilitative glucose transporter.
  • 组织特异性Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney.
  • 序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily perinuclear region in the absence of insulin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glucose transporter type 12 antibody
    • GLUT 12 antibody
    • GLUT-12 antibody
    • GLUT12 antibody
    • GLUT8 antibody
    • GTR12_HUMAN antibody
    • SLC2A12 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 12 antibody
    • Solute carrier family 2 facilitated glucose transporter member 12 antibody
    • Solute carrier family 2 member 12 antibody
    • Solute carrier family 2, facilitated glucose transporter member 12 antibody
    see all

Anti-GLUT12 antibody 图像

  • All lanes : Anti-GLUT12 antibody (ab100993) at 1 µg/ml

    Lane 1 : Human liver tissue lysate (cirrhosis) - membrane extract (ab29881)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab100993 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab100993 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GLUT12 antibody (ab100993)参考文献

ab100993 has not yet been referenced specifically in any publications.

Product Wall

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