Anti-GLUD1 + GLUD2抗体(ab99470)

概述

  • 产品名称Anti-GLUD1 + GLUD2抗体
    参阅全部 GLUD1 + GLUD2 一抗
  • 描述
    山羊多克隆抗体to GLUD1 + GLUD2
  • 特异性ab99470 is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to Human GLUD1 + GLUD2 aa 93-107 (internal sequence) (Cysteine residue). SwissProt: P49448 and P00367
    Sequence:

    ESEEQKRNRVRGILR

  • 阳性对照
    • Human and Mouse liver lysates.

性能

应用

Our Abpromise guarantee covers the use of ab99470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 61 kDa.

靶标

  • 相关性GLUD1: Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. GLUD2: Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
  • 数据库链接
  • 别名
    • GDH 1 antibody
    • GDH 2 antibody
    • GLUD antibody
    • GLUD1 antibody
    • GLUD2 antibody
    • GLUDP1 antibody
    • Glutamate dehydrogenase 1, mitochondrial antibody
    • Glutamate dehydrogenase 2, mitochondrial antibody
    see all

Anti-GLUD1 + GLUD2 antibody 图像

  • Anti-GLUD1 + GLUD2 antibody (ab99470) at 0.3 µg/ml + Mouse liver lysate at 35 µg

    Predicted band size : 61 kDa


  • Predicted band size : 61 kDa

    Western Blot of Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer) labelling GLUD1 + GLUD2 with ab99470 at a concentration of 0.01µg/ml for 1 hour.

  • EB09856 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex hows mitochondrial staining in neuronal cell bodies and axons. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-GLUD1 + GLUD2 antibody (ab99470)参考文献

ab99470 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99470.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"