概述
- 产品名称Anti-GLUD1抗体(Biotin)
参阅全部 GLUD1 一抗 - 描述兔多克隆抗体to GLUD1 (Biotin)
- 偶联物Biotin
- 特异性Due to high sequence homology with GLUD2 there is a potential for cross-reactivity.
- 经测试应用适用于: Dot blot, WB, ELISA, Immunomicroscopymore details
- 种属反应性与反应: Cow
- 免疫原
Full length native protein (purified) corresponding to Cow GLUD1. Full length native protein from Bovine Liver.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 -
Concentration information loading... - 纯度IgG fraction
- 纯化说明Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab34810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot | ||
| WB | ||
| ELISA | ||
| Immunomicroscopy |
ELISA: 1/4,000 - 1/20,000.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
This product has been assayed against 1.0 µg of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
- 功能May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
- 疾病相关Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
- 序列相似性Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
- 细胞定位Mitochondrion matrix.
- Information by UniProt
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数据库链接
- Entrez Gene: 281785 Cow
- SwissProt: P00366 Cow
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别名
- AI118167 antibody
- DHE3_HUMAN antibody
- EC 1.4.1.3 antibody
see all
Anti-GLUD1 antibody (Biotin) (ab34810)参考文献
ab34810 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"