The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Dot: Use at an assay dependent dilution.
ELISA: 1/4,000 - 1/20,000.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
This product has been assayed against 1.0 µg of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
疾病相关Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
序列相似性Belongs to the Glu/Leu/Phe/Val dehydrogenases family.