概述

  • 产品名称Anti-GLUD1抗体
    参阅全部 GLUD1 一抗
  • 描述
    兔多克隆抗体to GLUD1
  • 特异性Due to high sequence homology with GLUD2 there is a potential for cross-reactivity.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Saccharomyces cerevisiae, Chimpanzee, African Green Monkey
  • 免疫原

    Synthetic peptide corresponding to Human GLUD1 aa 72-121 (N terminal).
    Database link: P00367

  • 阳性对照
    • HT1080 cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human liver cancer.

性能

应用

Our Abpromise guarantee covers the use of ab89967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
  • 疾病相关Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
  • 序列相似性Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI118167 antibody
    • DHE3_HUMAN antibody
    • EC 1.4.1.3 antibody
    • GDH 1 antibody
    • GDH antibody
    • Gdh-X antibody
    • GDH1 antibody
    • GLUD antibody
    • Glud1 antibody
    • Glud1a antibody
    • Glud1b antibody
    • Gludl antibody
    • Glutamate dehydrogenase (NAD(P)+) antibody
    • Glutamate dehydrogenase 1 antibody
    • Glutamate dehydrogenase 1 mitochondrial antibody
    • Glutamate dehydrogenase 1a antibody
    • Glutamate dehydrogenase 1b antibody
    • Memory-related gene 2 protein antibody
    • MGC127177 antibody
    • MGC132003 antibody
    • MGC80801 antibody
    • MGC93608 antibody
    • mitochondrial antibody
    • MRG-2 antibody
    see all

Anti-GLUD1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancrease tissue labelling GLUD1 with ab89967.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling GLUD1 with ab89967.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney (cortex) tissue labelling GLUD1 with ab89967.

  • Anti-GLUD1 antibody (ab89967) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
  • IHC image of GLUD1 staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89967, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GLUD1 antibody (ab89967)参考文献

This product has been referenced in:
  • Broniarek I  et al. The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells. Pflugers Arch 468:1541-54 (2016). WB ; Human . Read more (PubMed: 27417103) »

See 1 Publication for this product

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