Anti-Glucose Transporter GLUT6抗体(ab119272)

概述

  • 产品名称Anti-Glucose Transporter GLUT6抗体
    参阅全部 Glucose Transporter GLUT6 一抗
  • 描述
    兔多克隆抗体to Glucose Transporter GLUT6
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Glucose Transporter GLUT6.

  • 阳性对照
    • This antibody gave a positive signal in Human Brain tissue lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

靶标

  • 功能Facilitative glucose transporter; binds cytochalasin B with low affinity.
  • 组织特异性Highly expressed in brain, spleen and peripheral blood leukocytes.
  • 序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 细胞定位Cell membrane. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A330096C23 antibody
    • F630103L12Rik antibody
    • Glucose transporter type 6 antibody
    • Glucose transporter type 9 antibody
    • GLUT-6 antibody
    • GLUT-9 antibody
    • Glut6 antibody
    • Glut9 antibody
    • GTR6_HUMAN antibody
    • HSA011372 antibody
    • RP23-449M10.2 antibody
    • SLC2A6 antibody
    • Solute carrier family 2, facilitated glucose transporter member 6 antibody
    see all

Anti-Glucose Transporter GLUT6 antibody 图像

  • Anti-Glucose Transporter GLUT6 antibody (ab119272) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa


    Exposure time : 8 minutes
  • ab119272 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119272 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glucose Transporter GLUT6 antibody (ab119272)参考文献

ab119272 has not yet been referenced specifically in any publications.

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