Anti-Glucose Transporter GLUT4抗体[mAbcam48547] (ab48547)
Key features and details
- Mouse monoclonal [mAbcam48547] to Glucose Transporter GLUT4
- Suitable for: WB
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-Glucose Transporter GLUT4抗体[mAbcam48547]
参阅全部 Glucose Transporter GLUT4 一抗 -
描述
小鼠单克隆抗体[mAbcam48547] to Glucose Transporter GLUT4 -
宿主
Mouse -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra) -
种属反应性
与反应: Mouse, Recombinant fragment
预测可用于: Rat, Sheep, Rabbit, Goat, Horse, Cow -
免疫原
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阳性对照
- WB: ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
mAbcam48547 -
骨髓瘤
Sp2/0 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48547于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa).
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说明 |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 54 kDa). |
靶标
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功能
Insulin-regulated facilitative glucose transporter. -
组织特异性
Skeletal and cardiac muscles; brown and white fat. -
疾病相关
Diabetes mellitus, non-insulin-dependent -
序列相似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻译后修饰
Sumoylated. -
细胞定位
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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数据库链接
- Entrez Gene: 282359 Cow
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- SwissProt: Q27994 Cow
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 10661 Mouse
- Unigene: 1314 Rat
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别名
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
图片
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Anti-Glucose Transporter GLUT4 antibody [mAbcam48547] (ab48547) at 5 µg/ml + Partial tagged recombinant protein to GLUT4 at 0.1 µg
Secondary
Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab48547 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab48547 被引用在 7 文献中.
- Panahi M et al. Insulin-Independent and Dependent Glucose Transporters in Brain Mural Cells in CADASIL. Front Genet 11:1022 (2020). PubMed: 33101365
- Ono E et al. Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment. PLoS One 13:e0195960 (2018). PubMed: 29677207
- Li J et al. Inhibition of insulin/PI3K/AKT signaling decreases adipose Sortilin 1 in mice and 3T3-L1 adipocytes. Biochim Biophys Acta Mol Basis Dis 1863:2924-2933 (2017). PubMed: 28844948
- Joannides CN et al. Dapagliflozin improves insulin resistance and glucose intolerance in a novel transgenic rat model of chronic glucose overproduction and glucose toxicity. Diabetes Obes Metab 19:1135-1146 (2017). PubMed: 28244693
- Mano Y et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . PubMed: 25125631
- Karim S et al. Dysregulated hepatic expression of glucose transporters in chronic disease: contribution of semicarbazide-sensitive amine oxidase to hepatic glucose uptake. Am J Physiol Gastrointest Liver Physiol 307:G1180-90 (2014). PubMed: 25342050
- Mattmiller SA et al. Glucose transporter and hypoxia-associated gene expression in the mammary gland of transition dairy cattle. J Dairy Sci 94:2912-22 (2011). PubMed: 21605761