Anti-Glucose Transporter GLUT4抗体(ab33780)

概述

  • 产品名称Anti-Glucose Transporter GLUT4抗体
    参阅全部 Glucose Transporter GLUT4 一抗
  • 描述
    兔多克隆抗体to Glucose Transporter GLUT4
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Rabbit, Goat, Horse, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Glucose Transporter GLUT4.

    (Peptide available as ab34088.)

  • 阳性对照
    • This antibody gave a positive signal in WB using partial recombinant protein to GLUT4 and the following human tissue lysates: Heart, Skeletal Muscle. This antibody gave a positive result in IHC in the following FFPE tissues: Human normal heart muscle, Mouse normal skeletal muscle.

性能

应用

Our Abpromise guarantee covers the use of ab33780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Insulin-regulated facilitative glucose transporter.
  • 组织特异性Skeletal and cardiac muscles; brown and white fat.
  • 疾病相关Diabetes mellitus, non-insulin-dependent
  • 序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰Sumoylated.
  • 细胞定位Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • 数据库链接
  • 别名
    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

Anti-Glucose Transporter GLUT4 antibody 图像

  • Immunohistochemical of PFA-fixed paraffin-embedded rat skeletal muscle skeletal tissue, labelling glucose transporter GLUT4 with ab33780 at a dilution of 1/200 incubated for 13 hours at 4°C in 1% BSA in TBS. Antigen retrival was with Tris-EDTA at pH 9.0 (heat mediated). Blocking was with 3% BSA incubated for 1 hour at 37°C. Secondary was a Goat anti-rabbit polyclonal Akaline Phosphotase conjugate at 1/200.

    See Abreview

  • IHC image of Glucose Transporter GLUT4 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33780, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Glucose Transporter GLUT4 antibody (ab33780) at 1 µg/ml

    Lane 1 : Recombinant Protein GLUT4 (Partial, Tagged) at 0.1 µg
    Lane 2 : Heart (Human) Tissue Lysate at 20 µg
    Lane 3 : Skeletal Muscle (Human) Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • IHC image of Glucose Transporter GLUT4 staining in Mouse normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33780, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab33780 staining Glucose Transporter GLUT4 in Mouse kidny tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 2% Horse serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor®568-conjugated Donkey anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

  • Anti-Glucose Transporter GLUT4 antibody (ab33780) at 1 µg/ml + Partial tagged recombinant protein to GLUT4 at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 55 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    ab33780 gave a positive signal against the partial recombinant GLUT4 protein which has an expected molecular weight of 30 kDa.

  • ab33780 staining glucose transporter GLUT4 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • ICC/IF image of ab33780 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33780, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glucose Transporter GLUT4 antibody (ab33780)参考文献

This product has been referenced in:
  • Liu L  et al. Effects of Noise Exposure on Systemic and Tissue-Level Markers of Glucose Homeostasis and Insulin Resistance in Male Mice. Environ Health Perspect 124:1390-8 (2016). IHC . Read more (PubMed: 27128844) »
  • Johansson J  et al. Electrical vs manual acupuncture stimulation in a rat model of polycystic ovary syndrome: different effects on muscle and fat tissue insulin signaling. PLoS One 8:e54357 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 23349861) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Skeletal muscle)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization No
Specification Skeletal muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (kidney)
Specification kidney
Fixative Acetone
Permeabilization No
Blocking step horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Mrs. Domenica McCarthy

Verified customer

提交于 Apr 08 2013

DISCOUNT CODE: xxxx
Expiration date: 23rd February 2013

I am very pleased to hear you would like to test ab33780 in IHC-frozen sections with your mouse tissue. This code will give you the value of ab33780 off your next order before the exp...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (A20 cell line, Mouse B cell lymphoma)
Specification A20 cell line, Mouse B cell lymphoma
Preparation Cell harvesting/tissue preparation method: suspension cells, so used directly.
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Fixation unfixed
Permeabilization No
Gating Strategy total stained cells
Username

Prof. Paul Townsend

Verified customer

提交于 May 07 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse Cell lysate - whole cell (mouse Cardiomyocyt)
Loading amount 20 µg
Specification mouse Cell lysate - whole cell (mouse Cardiomyocyt
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Prof. Paul Townsend

Verified customer

提交于 May 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human Cardiomyocytes)
Loading amount 20 µg
Specification Human Cardiomyocytes
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE gel with a 5% stacking gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Prof. Paul Townsend

Verified customer

提交于 Mar 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (MUSCLE, PROSTATE)
Loading amount 20 µg
Specification MUSCLE, PROSTATE
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 04 2010

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Aug 10 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Jul 22 2009

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