Anti-Glucose Transporter GLUT4抗体[1F8] (ab35826)

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概述

  • 产品名称
    Anti-Glucose Transporter GLUT4抗体[1F8]
    参阅全部 Glucose Transporter GLUT4 一抗
  • 描述
    小鼠单克隆抗体[1F8] to Glucose Transporter GLUT4
  • 宿主
    Mouse
  • 特异性
    This antibody is specific for the fat/muscle specific isoform of the Glucose Transporter GLUT4. We have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用
    适用于: ICC/IF, IP, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human, Monkey
  • 免疫原

    Rat Glucose Transporter GLUT4 from partially purified vesicles.

  • 表位
    This antibody has been shown to recognise an epitope in the cytoplasmic portion of the molecule.

性能

应用

Our Abpromise guarantee covers the use of ab35826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2.5 - 5 µg/ml.
IP Use at an assay dependent concentration. Membrane proteins at a concentration of 1-2 mg/ml can be immunoadsorbed using this antibody coupled with agarose beads.
IHC-Fr Use at an assay dependent concentration. PubMed: 20418694
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. 5 µg/ml of antibody will detect Glucose Transporter protein contained in 50-200 µg of solubilised membrane.

靶标

  • 功能
    Insulin-regulated facilitative glucose transporter.
  • 组织特异性
    Skeletal and cardiac muscles; brown and white fat.
  • 疾病相关
    Diabetes mellitus, non-insulin-dependent
  • 序列相似性
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰
    Sumoylated.
  • 细胞定位
    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

图片

  • Western blot - Anti-Glucose Transporter GLUT4 antibody [1F8] (ab35826)
    Western blot - Anti-Glucose Transporter GLUT4 antibody [1F8] (ab35826)
    3T3-L1 lysate probed with Mouse anti Rat GLUT4 (ab35826) followed by Goat anti Mouse IgG:Dylight®800. Imaged using the LiCor Odyssey ®

文献

This product has been referenced in:
  • Aimé P  et al. Modulation of olfactory sensitivity and glucose-sensing by the feeding state in obese Zucker rats. Front Behav Neurosci 8:326 (2014). IHC-Fr ; Rat . Read more (PubMed: 25278856) »
  • Reed SE  et al. A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. J Cell Sci 126:1931-41 (2013). Mouse . Read more (PubMed: 23444368) »

See all 6 Publications for this product

发表研究结果有使用 ab35826？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1 Review or Q&A

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Shortly, the customer wishes to know why ab35826 datasheet states samples should not be boiled prior to WB and is this a GLUT4 related or specific antibody related requirement. Thank you for your kind help. Regards,

Thank you for your enquiry regarding ab35826. I can confirm that ab35826 has been tested for Western blot application under reducing and denaturing conditions and the samples were boiled for 5 mins This antibody has not been characterized under n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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