Anti-Glucose Transporter GLUT3抗体(ab41525)


  • 产品名称Anti-Glucose Transporter GLUT3抗体
    参阅全部 Glucose Transporter GLUT3 一抗
  • 描述
    兔多克隆抗体to Glucose Transporter GLUT3
  • 经测试应用适用于: WB, IP, IHC-Fr, ELISA, Dot Blot, IHC-FrFl, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the C terminus of Glucose Transporter GLUT3.

  • 阳性对照
    • Rat brain, C12 cells, rat testicular germ cells



Our Abpromise guarantee covers the use of ab41525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/8000. Detects a band of approximately 45-47 kDa.Can be blocked with Glucose Transporter GLUT3 peptide (ab105625). Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
IP Use at an assay dependent concentration. Use at an assay dependent dilution. 2 ul ab41525 will immunoprecipitate 80-85% GLUT3 from 250 ug rat solublized hippocampal membranes
IHC-Fr 1/250.
ELISA 1/25000.
Dot Blot 1/25000. With solublized hippocampal plasma membranes.
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml.


Anti-Glucose Transporter GLUT3 antibody 图像

  • ab41525 staining GLUT3 in human duodenum.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab41525 at a 1/100 dilution staining Glucose Transporter GLUT3 in human PMN cells by Immunocytochemistry/ Immunofluorescence (PFA fixed), incubated for 4 hours at 37°C. Permeabilized using 0.1% Triton X-100 in 2% BSA for 15 minutes. Blocked with 2% BSA for 30 minutes at 22°C. Secondary used undiluted polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568 (red). Counterstain DAPI (blue).

    See Abreview

Anti-Glucose Transporter GLUT3 antibody (ab41525)参考文献

This product has been referenced in:
  • Krasznai ZT  et al. (18)FDG a PET tumor diagnostic tracer is not a substrate of the ABC transporter P-glycoprotein. Eur J Pharm Sci 64:1-8 (2014). Read more (PubMed: 25149126) »
  • Liu XS  et al. ZBTB7A acts as a tumor suppressor through the transcriptional repression of glycolysis. Genes Dev 28:1917-28 (2014). Read more (PubMed: 25184678) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain, heart, liver, muscle, retina)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 30 µg
Specification Brain, heart, liver, muscle, retina
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Aug 23 2016

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

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Thank you for contacting Abcam earlier today.

I am sorry about the issues you are having with ab41525 in western blot and as I mentioned before the antibody is covered under our Abpromise for six months and is guaranteed to work in western blo...

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Application Western blot
Sample Mouse Cell lysate - whole cell (Isolated Hippocampal neurons (E16))
Loading amount 100000 cells
Specification Isolated Hippocampal neurons (E16)
Gel Running Conditions Reduced Denaturing (15% SDS-PAGE Tris-Glycine)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Gregory O'Sullivan

Verified customer

提交于 Jun 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

提交于 May 24 2012

Vielen Dank für Ihre Anfrage und für Ihre Geduld.

Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer...

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Thank you for your participation in Abcam's post complaint survey.

I am sorry to hear that the replacement antibody ab68792 has not worked in mouse. Mouse is an untested species for this antibody and therefore, I had sent you also a discount ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your email.

The lot number for ab41525 you were using was: GR41399-4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your email.

Abreviews should reflect the customer's opinion. Thus, I will leave it up to you to include the competitor's antibody. To us, either way is fine. We do not edit reviews for such information, only for completeness so...

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