Anti-Glucose Transporter GLUT3抗体(ab41525)

概述

  • 产品名称
    Anti-Glucose Transporter GLUT3抗体
    参阅全部 Glucose Transporter GLUT3 一抗
  • 描述
    兔多克隆抗体to Glucose Transporter GLUT3
  • 经测试应用
    适用于: WB, IP, IHC-Fr, ELISA, Dot blot, IHC-FrFl, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the C terminus of Glucose Transporter GLUT3.

  • 阳性对照
    • IHC-P: Human duodenum tissue.

性能

应用

Our Abpromise guarantee covers the use of ab41525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/8000. Detects a band of approximately 45-47 kDa.Can be blocked with Glucose Transporter GLUT3 peptide (ab105625). Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.
IP Use at an assay dependent concentration.

Use at an assay dependent dilution. 2µl ab41525 will immunoprecipitate 80-85% GLUT3 from 250µg rat solublized hippocampal membranes.

IHC-Fr 1/250.
ELISA 1/25000.
Dot blot 1/25000. With solublized hippocampal plasma membranes.
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml.

靶标

Anti-Glucose Transporter GLUT3 antibody 图像

  • All lanes : Anti-Glucose Transporter GLUT3 antibody (ab41525) at 1/2000 dilution

    Lane 1 : Sal 3 month cortical extract
    Lane 2 : Glu 3 month cortical extract
    Lane 3 : CB 3 month cortical extract
    Lane 4 : Sal 3 month cortical extract
    Lane 5 : Glu 3 month cortical extract
    Lane 6 : GB 3 month cortical extract
    Lane 7 : Sal 3 month cortical extract
    Lane 8 : Glu 3 month cortical extract
    Lane 9 : CB 3 month cortical extract
    Lane 10 : Sal 3 month cortical extract
    Lane 11 : Glu 3 month cortical extract
    Lane 12 : CB 3 month cortical extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    Alkaline phosphatase-conjugated goat anti-rabbit IgG at 1/5000 dilution

    10% SDS-PAGE gel.

    Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.

  • ab41525 staining GLUT3 in human duodenum.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab41525 at a 1/100 dilution staining Glucose Transporter GLUT3 in human PMN cells by Immunocytochemistry/ Immunofluorescence (PFA fixed), incubated for 4 hours at 37°C. Permeabilized using 0.1% Triton X-100 in 2% BSA for 15 minutes. Blocked with 2% BSA for 30 minutes at 22°C. Secondary used undiluted polyclonal goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 568 (red). Counterstain DAPI (blue).

    See Abreview

Anti-Glucose Transporter GLUT3 antibody (ab41525)参考文献

This product has been referenced in:
  • Zhu Y  et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog 12:e1005648 (2016). WB ; Rat . Read more (PubMed: 27187079) »
  • Tu H  et al. Low Red Blood Cell Vitamin C Concentrations Induce Red Blood Cell Fragility: A Link to Diabetes Via Glucose, Glucose Transporters, and Dehydroascorbic Acid. EBioMedicine 2:1735-50 (2015). Mouse . Read more (PubMed: 26870799) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, heart, liver, muscle, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
30 µg
Specification
Brain, heart, liver, muscle, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2016

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

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Thank you for contacting Abcam earlier today.

I am sorry about the issues you are having with ab41525 in western blot and as I mentioned before the antibody is covered under our Abpromise for six months and is guaranteed to work in western blo...

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (Isolated Hippocampal neurons (E16))
Loading amount
100000 cells
Specification
Isolated Hippocampal neurons (E16)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE Tris-Glycine)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gregory O'Sullivan

Verified customer

提交于 Jun 14 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (RAW 264.7)
Specification
RAW 264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 May 24 2012

Vielen Dank für Ihre Anfrage und für Ihre Geduld.

Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer...

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Thank you for your participation in Abcam's post complaint survey.

I am sorry to hear that the replacement antibody ab68792 has not worked in mouse. Mouse is an untested species for this antibody and therefore, I had sent you also a discount ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your email.

The lot number for ab41525 you were using was: GR41399-4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your email.

Abreviews should reflect the customer's opinion. Thus, I will leave it up to you to include the competitor's antibody. To us, either way is fine. We do not edit reviews for such information, only for completeness so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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