Anti-Glucose Transporter GLUT3抗体(ab15311)

概述

性能

应用

Our Abpromise guarantee covers the use of ab15311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Facilitative glucose transporter. Probably a neuronal glucose transporter.
  • 组织特异性Highly expressed in brain. Expressed in many tissues.
  • 序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • brain antibody
    • FLJ90380 antibody
    • Glucose transporter type 3 antibody
    • Glucose transporter type 3 brain antibody
    • GLUT 3 antibody
    • GLUT-3 antibody
    • GLUT3 antibody
    • GTR3_HUMAN antibody
    • Slc2a3 antibody
    • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
    • Solute carrier family 2, facilitated glucose transporter member 3 antibody
    see all

Anti-Glucose Transporter GLUT3 antibody 图像

  • ab15311 staining Glucose Transporter GLUT3 in human placenta by Immunohistochemistry (FFPE-sections).
  • All lanes : Anti-Glucose Transporter GLUT3 antibody (ab15311) at 1/400 dilution

    Lane 1 : Lysate prepared from U87-MG cells
    Lane 2 : Lysate prepared from human brain
    Lane 3 : Lysate prepared from human brain
    Lane 4 : Lysate prepared from lymphoblastoid cell line

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat anti-rabbit IgG-HRP at 1/7500 dilution

    Predicted band size : 60 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa (possible glycosylated form).

    Image courtesy of Georg Ziegler by Abreview.

    See Abreview

Anti-Glucose Transporter GLUT3 antibody (ab15311)参考文献

This product has been referenced in:
  • Vittori A  et al. Copy-number variation of the neuronal glucose transporter gene SLC2A3 and age of onset in Huntington's disease. Hum Mol Genet N/A:N/A (2014). WB ; Human . Read more (PubMed: 24452335) »
  • McKinnon B  et al. Glucose transporter expression in eutopic endometrial tissue and ectopic endometriotic lesions. J Mol Endocrinol 52:169-79 (2014). Read more (PubMed: 24412827) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Human Tissue sections (Endometriosis)
Specification Endometriosis
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Brett McKinnon

Verified customer

提交于 Jun 10 2013

Thank you for your reply and for these additional details.

I can see no apparent reason why this product would be giving you this pattern with your rat cortex samples independent of age or treatment. It is especially strange to me that it is...

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Thank you for contacting us.

Your issue is very interesting as some animals because of the variablity between samples independent of age or treatment. Would you be able to provide your WB protocols and sample treatments? It may be an issue...

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Vielen Dank für Ihre Anfrage und für Ihre Geduld.

Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements.

To check the status of the order please contact our Customer Service team ...

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Vielen Dank für Ihre Anfrage. Es freut mich bestätigen zu können, dass beide Antikörper keine Lot-spezifische Konzentration aufweisen. ab14994 hat eine Konzentration von 1mg/ml und ab15311 hat eine Konzentration von 0.2mg/ml. Bitte folgen Sie diesen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U87-MG, human brain, lymphoblastoid cell-line)
Loading amount 15 µg
Specification U87-MG, human brain, lymphoblastoid cell-line
Gel Running Conditions Reduced Denaturing (4-12 % Bis-Tris Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Georg Ziegler

Verified customer

提交于 Jan 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (breast cancer cell lines)
Loading amount 30 µg
Specification breast cancer cell lines
Gel Running Conditions Reduced Denaturing (10% gel + glycerol)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Kristina Terrell

Verified customer

提交于 Aug 20 2010

Thank you for your enquiry. I was able to obtain the immunogen sequence. This has been updated on the datasheet: SIEPAKETTTNV aa 485-496 of human Unfortunately, it appears there is no significant homology between the chicken sequence and the imm...

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