Anti-Glucose Transporter GLUT2抗体(ab95256)

概述

  • 产品名称Anti-Glucose Transporter GLUT2抗体
    参阅全部 Glucose Transporter GLUT2 一抗
  • 描述
    兔多克隆抗体to Glucose Transporter GLUT2
  • 经测试应用适用于: ICC/IF, IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Glucose Transporter GLUT2.

    (Peptide available as ab105630.)

  • 阳性对照
    • This antibody gave a positive signal in Human Pancreas Tissue Lysate. It also gave a positive signal in FFPE human normal liver tissue sections.

应用

Our Abpromise guarantee covers the use of ab95256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
  • 组织特异性Liver, insulin-producing beta cell, small intestine and kidney.
  • 疾病相关Fanconi-Bickel syndrome
  • 序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • liver antibody
    • Glucose Transporter 2 antibody
    • Glucose Transporter GLUT2 antibody
    • Glucose transporter type 2 antibody
    • Glucose transporter type 2 liver antibody
    • Glucose transporter, liver/islet antibody
    • GLUT-2 antibody
    • GLUT2 antibody
    • GTR2_HUMAN antibody
    • GTT2 antibody
    • SLC2A2 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 2 antibody
    • Solute carrier family 2 facilitated glucose transporter member 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 2 antibody
    see all

Anti-Glucose Transporter GLUT2 antibody 图像

  • Anti-Glucose Transporter GLUT2 antibody (ab95256) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg/ml

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 8 minutes
  • ICC/IF image of ab95256 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95256, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.
  • IHC image of Glucose Transporter GLUT2 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95256, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glucose Transporter GLUT2 antibody (ab95256)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Western blot
Sample Baboon Tissue lysate - whole (Adult liver)
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Loading amount 25 µg
Treatment letrozole + estradiol benzoate (115 ug/kg body weight/day) in utero for 100 days
Specification Adult liver
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Soon-ok Kim

Verified customer

提交于 Aug 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Gel Running Conditions Reduced Denaturing (13)
Loading amount 40 µg
Specification Liver
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 28 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer x1 ab93678
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 04 2016

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Mouse Tissue sections (Pancreas)
Specification Pancreas
Permeabilization Yes - Tween20 0,1%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 02 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (small intestine)
Specification small intestine
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 18 2014

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative Acetone
Permeabilization No
Blocking step horse serum in 1XPBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Mrs. Domenica McCarthy

Verified customer

提交于 Apr 05 2013

Thank you for contacting us.

HEK293 cells can also be used as positive control.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

DISCOUNT CODE: xxxx
Expiration date: 23rd February 2013

I am very pleased to hear you would like to test ab395256 in IHC-frozen sections with your mouse tissue. This code will give you the value of ab395256 off your next order before the e...

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Thank you for your reply and also for checking the lysate. This is really helpful.

I am sorry to hear that the problem has not been resolved.
Since you see no protein bands before transfer, it does not surprise me that you see none after...

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