Anti-Glucose Transporter GLUT2抗体[5D1] (ab85715)
Key features and details
- Mouse monoclonal [5D1] to Glucose Transporter GLUT2
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Glucose Transporter GLUT2抗体[5D1]
参阅全部 Glucose Transporter GLUT2 一抗 -
描述
小鼠单克隆抗体[5D1] to Glucose Transporter GLUT2 -
宿主
Mouse -
特异性
ab85715 recognizes Glucose Transporter GLUT2 expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the Human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3. -
经测试应用
适用于: IHC-P, Flow Cyt, WBmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human Glucose Transporter GLUT2. Recombinant protein corresponding to Met1-Val524 from human GLUT 2 expressed in NS0 cells.
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阳性对照
- Liver tissue. Flow Cytometry: HepG2 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituents: PBS, 5% Trehalose -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
5D1 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85715于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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说明 |
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IHC-P
Use a concentration of 10 µg/ml. |
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
靶标
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功能
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. -
组织特异性
Liver, insulin-producing beta cell, small intestine and kidney. -
疾病相关
Fanconi-Bickel syndrome -
序列相似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻译后修饰
N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6514 Human
- Omim: 138160 Human
- SwissProt: P11168 Human
- Unigene: 167584 Human
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别名
- liver antibody
- Glucose Transporter 2 antibody
- Glucose Transporter GLUT2 antibody
see all
图片
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Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 8 minutes -
ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.
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Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab85715 被引用在 9 文献中.
- Pittala S et al. The VDAC1-based R-Tf-D-LP4 Peptide as a Potential Treatment for Diabetes Mellitus. Cells 9:N/A (2020). PubMed: 32093016
- Rogers RC et al. Evidence that hindbrain astrocytes in the rat detect low glucose with a glucose transporter 2-phospholipase C-calcium release mechanism. Am J Physiol Regul Integr Comp Physiol 318:R38-R48 (2020). PubMed: 31596114
- Villalva-Pérez JM et al. Characterization of Huh7 cells after the induction of insulin resistance and post-treatment with metformin. Cytotechnology 72:499-511 (2020). PubMed: 32409919
- Fujino M et al. Expression of glucose transporter-1 is correlated with hypoxia-inducible factor 1a and malignant potential in pancreatic neuroendocrine tumors. Oncol Lett 12:3337-3343 (2016). IHC-P ; Human . PubMed: 27900001
- Nguyen NQ et al. Upregulation of intestinal glucose transporters after Roux-en-Y gastric bypass to prevent carbohydrate malabsorption. Obesity (Silver Spring) 22:2164-71 (2014). PubMed: 24990218
- Mano Y et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . PubMed: 25125631
- Kern M et al. Liver-restricted Repin1 deficiency improves whole-body insulin sensitivity, alters lipid metabolism, and causes secondary changes in adipose tissue in mice. Diabetes 63:3295-309 (2014). PubMed: 24760138
- Deane AM et al. The effects of critical illness on intestinal glucose sensing, transporters, and absorption. Crit Care Med 42:57-65 (2014). PubMed: 23963126
- Vargas N et al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). IHC-FoFr ; Mouse . PubMed: 21857924