Anti-Glucose Transporter GLUT10抗体(ab110528)

概述

  • 产品名称Anti-Glucose Transporter GLUT10抗体
    参阅全部 Glucose Transporter GLUT10 一抗
  • 描述
    兔多克隆抗体to Glucose Transporter GLUT10
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Glucose Transporter GLUT10.

  • 阳性对照
    • This antibody gave positive in Human Pancreas tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.

性能

应用

Our Abpromise guarantee covers the use of ab110528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Facilitative glucose transporter.
  • 组织特异性Widely expressed; highest levels in liver and pancreas.
  • 疾病相关Defects in SLC2A10 are the cause of arterial tortuosity syndrome (ATS) [MIM:208050]. ATS is an autosomal recessive disorder characterized by tortuosity and elongation of major arteries, often resulting in death at young age. Other typical features include aneurysms of large arteries and stenosis of the pulmonary artery, in association with facial features and several connective tissue manifestations such as soft skin and joint laxity. Histopathological findings include fragmentation of elastic fibers in the tunica media of large arteries.
  • 序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 细胞定位Endomembrane system. Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA450473 antibody
    • facilitated glucose transporter member 10 antibody
    • Glucose transporter type 10 antibody
    • glut 10 antibody
    • GLUT-10 antibody
    • GTR10_HUMAN antibody
    • MGC126706 antibody
    • slc2a10 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 10 antibody
    see all

Anti-Glucose Transporter GLUT10 antibody 图像

  • IHC image of Glucose Transporter GLUT10 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110528, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Glucose Transporter GLUT10 antibody (ab110528) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-Glucose Transporter GLUT10 antibody (ab110528)参考文献

ab110528 has not yet been referenced specifically in any publications.

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