RabMAb

Anti-Glucose Transporter GLUT1抗体[EPR3915] (ab115730)

Abreviews (8)Q&A (5)Specific References (14)
Customer reviews and FAQs

概述

  • 产品名称Anti-Glucose Transporter GLUT1抗体[EPR3915]
    参阅全部 Glucose Transporter GLUT1 一抗
  • 描述
    兔单克隆抗体[EPR3915] to Glucose Transporter GLUT1
  • 经测试应用适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glucose Transporter GLUT1 aa 450 to the C-terminus.
    (Peptide available as ab202335)

  • 阳性对照
    • Jurkat, HepG2, 3T3L1, Mouse brain, Human fetal brain and Human fetal liver lysates; Human cervical carcinoma and colonic adenocarcinoma tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab115730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/500.
WB 1/100000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 54 kDa).Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).

We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)
Flow Cyt 1/40.

For unpurified, use 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • 组织特异性Expressed at variable levels in many human tissues.
  • 疾病相关Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • 序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Anti-Glucose Transporter GLUT1 antibody [EPR3915] 图像

  • Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/100000 dilution (purified)

    Lane 1 : NIH/3T3 whole cell lysate
    Lane 2 : PC-12 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 54 kDa
    Observed band size : 40-60 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemical staining of paraffin embedded rat kidney with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Flow Cytometry - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Flow Cytometry - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab115730 at a dilution of 1/40 (red line). The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Immunofluorescence staining of HepG2 cells with purified ab115730 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab115730 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemical staining of paraffin embedded mouse liver with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000000 dilution (purified)

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : HT-29 whole cell lysate
    Lane 4 : SW480 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 54 kDa
    Observed band size : 40-60 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000 dilution (Unpurified)

    Lane 1 : Jurkat lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : 3T3L1 lysate
    Lane 5 : Human fetal liver lysate
    Lane 6 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 54 kDa
  • Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Western blot - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/200000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : 3T3-L1 brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 54 kDa
    Observed band size : 40-60 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Flow Cytometry - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Overlay histogram showing HeLa cells stained with unpurified ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human cervical carcinoma tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human colonic adenocarcinoma tissue by Immunohistochemistry.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 showing positive staining in normal liver tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 showing positive staining in normal breast tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 showing positive staining in normal colon tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 showing positive staining in kidney carcinoma tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 showing negative staining in skeletal muscle tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 showing positive staining in urinary bladder transitional carcinoma tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)

    Unpurified ab115730 showing negative staining in normal heart tissue.

  • Other - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Other - Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)参考文献

This product has been referenced in:
  • Chen K  et al. Regulation of glucose metabolism by p62/SQSTM1 through HIF1a. J Cell Sci 129:817-30 (2016). Read more (PubMed: 26743088) »
  • Zhu Y  et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog 12:e1005648 (2016). WB, IHC-P ; Rat . Read more (PubMed: 27187079) »

See all 14 Publications for this product

发表研究结果有使用 ab115730？请让我们知道，以便我们可以引用本数据表中的参考文章。

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13 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (Retina)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 20 µg
Specification Retina
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Oct 04 2016

Western blot abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Excellent
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain, heart, liver, muscle)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 20 µg
Specification Mouse brain, heart, liver, muscle
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
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Abcam user community

Verified customer

提交于 Oct 03 2016

Western blot abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Excellent
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Jul 25 2016

Western blot abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Excellent
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification HUVEC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Jul 25 2016

I am looking for a blocking peptide for Ab115730, as a control for the specificity of it's use in IHC.

Thanks for your patience. The specific blocking peptide has been added as catalog number ab202335.



Please let me know if you have any other questions or concerns.

Western blot abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Below Average
Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12.5)
Sample Chinese Hamster Cell lysate - whole cell (ovary)
Specification ovary
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Daniel Wang

Verified customer

提交于 Feb 24 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Good
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Sample Rat Tissue sections (Liver cancer)
Specification Liver cancer
Permeabilization Yes - Wash Buffer from Dako with tween
Fixative Paraformaldehyde
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Mr. Rudolf Jung

Verified customer

提交于 Mar 19 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with tween
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Mr. Rudolf Jung

Verified customer

提交于 Apr 16 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT1 antibody [EPR3915]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with tween
Read More
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Mr. Rudolf Jung

Verified customer

提交于 Apr 16 2013



Inquiry: What is the epitope for GLUT1 antibody 115730? Or is it intra- or extra cellular?

Thank you for contacting us. The epitipe for the GLU1 antibody ab115730 is intracellular.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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