Anti-Glucose Transporter GLUT1抗体[EPR3915] (ab115730)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST

Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab115730 at a dilution of 1/40 (red line). The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

Immunofluorescence staining of HepG2 cells with purified ab115730 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab115730 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST

Overlay histogram showing HeLa cells stained with unpurified ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human cervical carcinoma tissue by Immunohistochemistry.

Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human colonic adenocarcinoma tissue by Immunohistochemistry.

Unpurified ab115730 showing positive staining in normal liver tissue.

Unpurified ab115730 showing positive staining in normal breast tissue.

Unpurified ab115730 showing positive staining in normal colon tissue.

Unpurified ab115730 showing positive staining in kidney carcinoma tissue.

Unpurified ab115730 showing negative staining in skeletal muscle tissue.

Unpurified ab115730 showing positive staining in urinary bladder transitional carcinoma tissue.

Unpurified ab115730 showing negative staining in normal heart tissue.