Glucose Detection试剂盒II (ab102517)


  • 产品名称Glucose Detection试剂盒II
    参阅全部 Glucose 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Quantitative
  • 灵敏度
    > 0.02 mM
  • 范围
    0.02 mM - 10 mM
  • 检测时间
    0h 30m
  • 产品概述

    Glucose Detection Kit II (ab102517) provides direct measurement of glucose in various biological samples, and it is particularly suitable for serum and urine samples since it is unaffected by reducing substances, which can interfere with detection in oxidase-based kits. In this assay, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit can detect glucose concentrations in the range of 20µM-10mM.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Glucose is an important fuel source to generate the universal energy molecule ATP. Serum glucose level is a key diagnostic parameter for many metabolic disorders.

  • 经测试应用适用于: Functional Studiesmore details


  • 相关性Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.


Our Abpromise guarantee covers the use of ab102517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Glucose Detection Kit II 图像

  • Glucose measured in biological fluids. Human samples diluted 20-80 fold. Mouse samples diluted 1-27 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Standard curve for glucose run using the kit protocol


Glucose Detection Kit II (ab102517)参考文献

ab102517 has not yet been referenced specifically in any publications.

Product Wall

The main difference between these two glucose assays is that in ab65333 the probe reacts with hydrogen peroxide and gets oxidized to produce colored compound detectable at 570nm or at Ex/Em 535/587nm. In ab102517, the probe reacts with NADH (produced d...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm I would suggest Glucose Detection Kit ab102517or ab65333 Glucose Assay Kitswill be suitable for detection andquantification of glucose in the c...

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Thakn you for your enquiry.
I am pleased to provide the further information below regarding the difference between these kits:


Absorbance 570 nm or Fluorescence Ex/Em 535/587 nm


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