Glucose Dehydrogenase Assay试剂盒(Colorimetric) (ab102532)

概述

  • 产品名称Glucose Dehydrogenase Assay试剂盒(Colorimetric)
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • 检测类型Enzyme activity
  • 灵敏度
    > 0.01 mU/well
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Glucose Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (? = 450 nm).
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    This assay detects GDH activity as low as 0.01 mU.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Glucose dehydrogenase (NAD+) (EC 1.1.1.118) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ = D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
  • 别名
    • GDH

应用

Our Abpromise guarantee covers the use of ab102532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Glucose Dehydrogenase Assay Kit (Colorimetric) 图像

  • Example of NADH standard curve obtained using ab102532
  • Example of Bovine Liver Sample test curves obtained using ab102532

实验方案

Glucose Dehydrogenase Assay Kit (Colorimetric) (ab102532)参考文献

ab102532 has not yet been referenced specifically in any publications.

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