Glucose Dehydrogenase Assay试剂盒(Colorimetric) (ab102532)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
概述
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产品名称
Glucose Dehydrogenase Assay试剂盒(Colorimetric) -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media -
检测类型
Enzyme activity -
灵敏度
> 0.01 mU/well -
检测时间
2h 00m -
产品概述
Abcam's Glucose Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm).
Visit our FAQs page for tips and troubleshooting. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K786 Glucose Dehydrogenase Activity Colorimetric Assay Kit. K786-100 is the same size as the 100 test size of ab102532.
This assay detects GDH activity as low as 0.01 mU.
Developer (Lyophilized) is light sensitive and should be protected from light during storage and preparation.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Developer (Lyophilized) 1 vial GDH Assay Buffer 1 x 25ml GDH Positive Control (Lyophilized) 1 vial Glucose 1 x 1ml NADH Standard (Lyophilized) 1 vial -
研究领域
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相关性
Glucose dehydrogenase (NAD+) (EC 1.1.1.118) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ = D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. -
别名
- GDH
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab102532 被引用在 1 文献中.
- Yalçin A et al. PKR inhibitors suppress endoplasmic reticulum stress and subdue glucolipotoxicity-mediated impairment of insulin secretion in pancreatic beta cells. Turk J Biol 44:93-102 (2020). PubMed: 32256145