Anti-Glucose 6 phosphate isomerase抗体(ab86950)

概述

  • 产品名称Anti-Glucose 6 phosphate isomerase抗体
    参阅全部 Glucose 6 phosphate isomerase 一抗
  • 描述
    兔多克隆抗体to Glucose 6 phosphate isomerase
  • 经测试应用适用于: ICC/IF, IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Cow, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Glucose 6 phosphate isomerase.

    (Peptide available as ab86951.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Rat Heart Rat Lung Human Heart

性能

应用

Our Abpromise guarantee covers the use of ab86950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use a concentration of 5 µg/ml.

靶标

  • 功能Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • 通路Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • 疾病相关Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • 序列相似性Belongs to the GPI family.
  • 翻译后修饰Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMF antibody
    • Aurocrine motility factor antibody
    • Autocrine motility factor antibody
    • DKFZp686C13233 antibody
    • EC 5.3.1.9 antibody
    • G6PI_HUMAN antibody
    • Glucose phosphate isomerase antibody
    • Glucose-6-phosphate isomerase antibody
    • GNPI antibody
    • GPI antibody
    • Gpi1 antibody
    • Hexose monophosphate isomerase antibody
    • Hexosephosphate isomerase antibody
    • Neuroleukin antibody
    • NLK antibody
    • Oxoisomerase antibody
    • PGI antibody
    • PHI antibody
    • Phosphoglucose isomerase antibody
    • Phosphohexomutase antibody
    • Phosphohexose isomerase antibody
    • Phosphosaccharomutase antibody
    • SA 36 antibody
    • SA-36 antibody
    • SA36 antibody
    • Sperm antigen 36 antibody
    see all

Anti-Glucose 6 phosphate isomerase antibody 图像

  • All lanes : Anti-Glucose 6 phosphate isomerase antibody (ab86950) at 1 µg/ml

    Lane 1 : Heart (Rat) Tissue Lysate
    Lane 2 : Lung (Rat) Tissue Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ICC/IF image of ab86950 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86950, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.
  • IHC image of Glucose 6 phosphate isomerase staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86950, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-Glucose 6 phosphate isomerase antibody (ab86950) at 1 µg/ml

    Lane 1 : Recombinant Human Glucose 6 phosphate isomerase protein (ab87625) at 0.1 µg
    Lane 2 : Recombinant Human Glucose 6 phosphate isomerase protein (ab87625) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds
  • Glucose 6 phosphate isomerase was immunoprecipitated using 0.5mg Rat Heart tissue lysate, 5µg of Rabbit polyclonal to Glucose 6 phosphate isomerase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Rat Heart tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab86950.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 63kDa; Glucose 6 phosphate isomerase

Anti-Glucose 6 phosphate isomerase antibody (ab86950)参考文献

This product has been referenced in:
  • Du Y  et al. Lysine malonylation is elevated in type 2 diabetic mouse models and enriched in metabolic associated proteins. Mol Cell Proteomics 14:227-36 (2015). Read more (PubMed: 25418362) »
  • Senesi S  et al. Contribution of fructose-6-phosphate to glucocorticoid activation in the endoplasmic reticulum: possible implication in the metabolic syndrome. Endocrinology 151:4830-9 (2010). Rat . Read more (PubMed: 20826560) »

See all 2 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (HEK293 cells)
Specification HEK293 cells
Total protein in input 500 µg
Username

Abcam user community

Verified customer

提交于 Aug 28 2014

Application Western blot
Sample Mouse Tissue lysate - other (Hippocampal cytosolic fractions)
Loading amount 60 µg
Specification Hippocampal cytosolic fractions
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gabriele Ebling Mattos

Verified customer

提交于 Dec 11 2012

It is absolutely fine to ask when it's not clear:

Steps 1-4 are correct. Step 5 isn't : as we cannot guarantee that the antibody works, we give you this discount code.

"The code will become active" means that in both cases (...

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Thank you for contacting us again.

No, to test the antibody, and to review the antibody, you have to order it first. Otherwise it would be a bit crystal globe reading, if the antibody works in your species. :)

But seriously, the Abrev...

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I am very pleased to hear you would like to accept our offer and test ab86950 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code...

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Thank you for your email.

As I wrote in my previous email,I would need to know in which application you want to use the antibody on mouse samples. If it is an application which is reviewable via our system, i can issue you a discount code.
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Thank you for contacting us.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, whi...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Username

Dr. Sophie Pezet

Verified customer

提交于 May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

提交于 Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"