Anti-Glucose 6 Phosphate Dehydrogenase抗体[AT2F6] (ab91034)

概述

  • 产品名称
    Anti-Glucose 6 Phosphate Dehydrogenase抗体[AT2F6]
    参阅全部 Glucose 6 Phosphate Dehydrogenase 一抗
  • 描述
    小鼠单克隆抗体[AT2F6] to Glucose 6 Phosphate Dehydrogenase
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat, Cow, Human
  • 免疫原

    Recombinant fragment, corresponding to internal sequence amino acids 35-506 of Human Glucose 6 Phosphate Dehydrogenase, purified from E. coli.

  • 阳性对照
    • MCF7 cell lysate
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab91034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 59 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能
    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 组织特异性
    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • 通路
    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 疾病相关
    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 序列相似性
    Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻译后修饰
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

图片

  • Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034) at 1/1000 dilution + MCF7 cell lysate at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP

    Developed using the ECL technique.

    Predicted band size: 59 kDa

实验方案

文献

ab91034 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Tissue lysate - whole (Bovine Coronary Artery)
Loading amount
35 µg
Specification
Bovine Coronary Artery
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

hirotaka ata

Verified customer

提交于 Apr 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (A7r5)
Loading amount
45 µg
Specification
A7r5
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

hirotaka ata

Verified customer

提交于 Apr 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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