Anti-Glucose 6 Phosphate Dehydrogenase抗体(ab76598)
- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-Glucose 6 Phosphate Dehydrogenase抗体
参阅全部 Glucose 6 Phosphate Dehydrogenase 一抗 -
描述兔多克隆抗体to Glucose 6 Phosphate Dehydrogenase
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宿主Rabbit
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经测试应用适用于: ICC/IF, IHC-P, WBmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat, Sheep, Cow, Chimpanzee, Baboon, Chinese hamster
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免疫原
Synthetic peptide derived from within residues 50 - 150 of Human Glucose 6 Phosphate Dehydrogenase.
(Peptide available as ab87367.)
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阳性对照
- This antibody gave a positive signal in RAW 264.7 and Jurkat whole cell lysates, and in human placenta and lymph node tissue lysates. IHC-P: Hodgkin's lymphoma
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab76598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
靶标
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功能Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
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组织特异性Isoform Long is found in lymphoblasts, granulocytes and sperm.
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通路Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
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疾病相关Anemia, non-spherocytic hemolytic, due to G6PD deficiency
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序列相似性Belongs to the glucose-6-phosphate dehydrogenase family.
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翻译后修饰Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
- Information by UniProt
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数据库链接
- Entrez Gene: 281179 Cow
- Entrez Gene: 2539 Human
- Entrez Gene: 14381 Mouse
- Entrez Gene: 24377 Rat
- Entrez Gene: 443211 Sheep
- Omim: 305900 Human
- SwissProt: P11413 Human
- SwissProt: Q00612 Mouse
see all -
别名
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598)ICC/IF image of ab76598 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76598, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598)IHC image of Glucose 6 Phosphate staining in human Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76598, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598)All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 30 seconds
实验方案
文献
This product has been referenced in:
- Chhabra A et al. Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4:6 (2018). Read more (PubMed: 29531803) »
- Wang J et al. Overexpression of G6PD is associated with poor clinical outcome in gastric cancer. Tumour Biol 33:95-101 (2012). IHC-P ; Human . Read more (PubMed: 22012600) »
客户评价及客户问答
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