Anti-Glucose 6 Phosphate Dehydrogenase抗体(ab76598)

概述

  • 产品名称
    Anti-Glucose 6 Phosphate Dehydrogenase抗体
    参阅全部 Glucose 6 Phosphate Dehydrogenase 一抗
  • 描述
    兔多克隆抗体to Glucose 6 Phosphate Dehydrogenase
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Cow, Chimpanzee, Baboon, Chinese hamster
  • 免疫原

    Synthetic peptide derived from within residues 50 - 150 of Human Glucose 6 Phosphate Dehydrogenase.

    (Peptide available as ab87367.)

  • 阳性对照
    • This antibody gave a positive signal in RAW 264.7 and Jurkat whole cell lysates, and in human placenta and lymph node tissue lysates. IHC-P: Hodgkin's lymphoma

性能

应用

Our Abpromise guarantee covers the use of ab76598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

靶标

  • 功能
    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 组织特异性
    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • 通路
    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 疾病相关
    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 序列相似性
    Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻译后修饰
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

图片

  • ICC/IF image of ab76598 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76598, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.
  • IHC image of Glucose 6 Phosphate staining in human Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76598, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab76598) at 1 µg/ml

    Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa


    Exposure time : 30 seconds

文献

This product has been referenced in:
  • Wang J  et al. Overexpression of G6PD is associated with poor clinical outcome in gastric cancer. Tumour Biol 33:95-101 (2012). IHC-P ; Human . Read more (PubMed: 22012600) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate boiling
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 29 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (A549 Lung cancer cells)
Specification
A549 Lung cancer cells
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Mar 04 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Loading amount
10 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Sep 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HMEC, MCF-10A, LN229, MDA-MB231 cell lines)
Loading amount
10 µg
Specification
HMEC, MCF-10A, LN229, MDA-MB231 cell lines
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. barbara chaneton

Verified customer

提交于 Jan 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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