Anti-Glucose 6 Phosphate Dehydrogenase抗体(ab61704)

概述

  • 产品名称Anti-Glucose 6 Phosphate Dehydrogenase抗体
    参阅全部 Glucose 6 Phosphate Dehydrogenase 一抗
  • 描述
    山羊多克隆抗体to Glucose 6 Phosphate Dehydrogenase
  • 特异性This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: a wide range of other species
  • 免疫原

    Synthetic peptide:

    C-KPASTNSDDVRDEK

    , corresponding to internal sequence amino acids 275-288 of Human Glucose 6 Phosphate Dehydrogenase

  • 阳性对照
    • Human Placenta lysate.

性能

应用

Our Abpromise guarantee covers the use of ab61704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA Use at an assay dependent dilution. (Peptide ELISA: antibody detection limit dilution 1:8,000)

靶标

  • 功能Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 组织特异性Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • 通路Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 疾病相关Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 序列相似性Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻译后修饰Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

Anti-Glucose 6 Phosphate Dehydrogenase antibody 图像

  • Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab61704) + Lysates prepared from Human Peripheral Blood Mononucleocytes at 35 µg

    Predicted band size : 60 kDa

Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab61704)参考文献

ab61704 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HMEC, MCF-10A, LN229, MDA-MB231 cell lines)
Loading amount 10 µg
Specification HMEC, MCF-10A, LN229, MDA-MB231 cell lines
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. barbara chaneton

Verified customer

提交于 Jan 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"