存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab106810 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 59 kDa.Can be blocked with
Glucose 6 Phosphate Dehydrogenase peptide (ab196304).
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 5 - 10 µg/ml. Increasing amount of recombinant G6PD captured by a rabbit antibody. ab106810 used as reporter antibody.
功能 Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
组织特异性 Isoform Long is found in lymphoblasts, granulocytes and sperm.
通路 Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
疾病相关 Anemia, non-spherocytic hemolytic, due to G6PD deficiency
序列相似性 Belongs to the glucose-6-phosphate dehydrogenase family.
翻译后修饰 Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
Information by UniProt
Anti-Glucose 6 Phosphate Dehydrogenase antibody 图像
Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab106810)
Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810) at 0.03 µg/ml + Human testis lysate (in RIPA buffer) at 35 µg developed using the ECL technique Predicted band size : 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810)
ab106810, at 2.5 µg/ml, staining Glucose 6 phosphate dehydrogenase in paraffin-embedded Human testis tissue by Immunohistochemistry using AP staining.
Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"