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Abcam's Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923) is a simple, sensitive and rapid means of quantifying glucose-6-Phosphate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). This assay can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. This Glucose-6-Phosphate Assay Kit - High Sensitivity is more sensitive than our colorimetric assay, Glucose-6-Phosphate Assay Kit (ab83426).
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Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
|Glucose 6 phosphate assay buffer||WM||1 x 25ml|
|Glucose 6 phosphate Enzyme Mix (lyophilized)||Green||1 vial|
|Glucose 6 phosphate Standard (10 µmol; lyophilized)||Yellow||1 vial|
|Glucose 6 phosphate Substrate Mix (lyophilized)||Red||1 vial|
|Probe (in DMSO)||Blue||1 x 0.4ml|
Our Abpromise guarantee covers the use of ab107923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Glucose 6 phosphate measured in mouse tissue lysates showing quantity (pmol) per mg protein of tested sample
Glucose 6 phosphate measured in biological fluids showing quantity (pmol) per microliter of tested sample
ab107923 has not yet been referenced specifically in any publications.