Glucose-6-Phosphate Assay试剂盒- High Sensitivity (Fluorometric) (ab107923)

概述

  • 产品名称Glucose-6-Phosphate Assay试剂盒- High Sensitivity (Fluorometric)
    参阅全部 Glucose 6 phosphate 试剂盒
  • 检测方法Fluorescent
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Quantitative
  • 灵敏度
    > 1 µM
  • 范围
    1 µM - 500 µM
  • 检测时间
    0h 20m
  • 产品概述

    Abcam's Glucose-6-Phosphate Assay Kit  - High Sensitivity (Fluorometric) (ab107923) is a simple, sensitive and rapid means of quantifying glucose-6-Phosphate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). This assay can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. This Glucose-6-Phosphate Assay Kit - High Sensitivity is more sensitive than our colorimetric assay, Glucose-6-Phosphate Assay Kit (ab83426).
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

  • 相关性Glucose 6 phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
  • 别名
    • G6P

应用

Our Abpromise guarantee covers the use of ab107923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) 图像

  • Glucose 6 phosphate measured in mouse tissue lysates showing quantity (pmol) per mg protein of tested sample

  • Glucose 6 phosphate measured in biological fluids showing quantity (pmol) per microliter of tested sample

  • Glucose 6 phosphate standard curves (0 – 500 and 0 – 50 pmol range) generated using ab107923.

实验方案

Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923)参考文献

ab107923 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Yes the assay buffer in the kit may be used. You may sonicate the cells being careful that they are fully lysed.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"