Anti-Glucokinase抗体- N-terminal (ab37796)

概述

  • 产品名称
    Anti-Glucokinase抗体- N-terminal
    参阅全部 Glucokinase 一抗
  • 描述
    兔多克隆抗体to Glucokinase - N-terminal
  • 经测试应用
    适用于: ELISA, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig, Common marmoset
  • 免疫原

    Synthetic peptide corresponding to Human Glucokinase (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P35557
    (Peptide available as ab108095)

  • 阳性对照
    • Human breast carcinoma and hepatocarcinoma tissues; HepG2 and mouse liver tissue cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab37796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 52 kDa).Can be blocked with Glucokinase peptide (ab108095).
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • 组织特异性
    Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • 疾病相关
    Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • 序列相似性
    Belongs to the hexokinase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP:D-hexose 6-phosphotransferase antibody
    • FGQTL3 antibody
    • GCK antibody
    • GK antibody
    • GLK antibody
    • Glucokinase antibody
    • Hexokinase D pancreatic isozyme antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HHF3 antibody
    • HK IV antibody
    • HK4 antibody
    • HKIV antibody
    • HXK4_HUMAN antibody
    • HXKP antibody
    • LGLK antibody
    • MODY2 antibody
    see all

图片

  • All lanes : Anti-Glucokinase antibody - N-terminal (ab37796)

    Lane 1 : Hep G2 cell lysate
    Lane 2 : Mouse liver lysate


    Predicted band size : 52 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • Formalin fixed, paraffin embedded human breast carcinoma tissue using ab37796, followed by AEC staining.
  • ICC/IF image of ab37796 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37796, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Ofori JK  et al. Elevated miR-130a/miR130b/miR-152 expression reduces intracellular ATP levels in the pancreatic beta cell. Sci Rep 7:44986 (2017). Read more (PubMed: 28332581) »
  • Kucejova B  et al. Hepatic mTORC1 Opposes Impaired Insulin Action to Control Mitochondrial Metabolism in Obesity. Cell Rep 16:508-19 (2016). Read more (PubMed: 27346353) »

See all 12 Publications for this product

客户评价及客户问答

The current lot we have in stock for ab37796 is GR8879-26. This has a concentration of 2 mg/ml.

Once you receive your order, if you have a different lot please do not hesitate to let me know and we can check the concentration for you.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Cell lysate - whole cell (HepG2)
Specification
HepG2
Treatment
+/- Testosterone
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 06 2013

Thank you for contacting us and for reporting the problem you experienced with the antibody ab37796. We will use the data you provided to investigate the source of the problem with this product. I am sorry that the product you received did not perfo...

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Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps test...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (pancreatic beta cells)
Loading amount
20 µg
Specification
pancreatic beta cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Sarah Ronnebaum

Verified customer

提交于 Jun 19 2007

Thank you for your patience in this matter. I have received a reply from the originator of ab37796 (IGLK antibody). Glucokinase is actually expressed in HepG2, please follow the links below to a couple of articles that demonstrate this point: h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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