Anti-Glucokinase抗体(ab88056)

概述

  • 产品名称Anti-Glucokinase抗体
    参阅全部 Glucokinase 一抗
  • 描述
    兔多克隆抗体to Glucokinase
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Cow, Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Human Glucokinase aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P35557
    (Peptide available as ab101298)

  • 阳性对照
    • This antibody gave a positive signal in human and mouse liver tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab88056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

靶标

  • 功能Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • 组织特异性Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • 疾病相关Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • 序列相似性Belongs to the hexokinase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP:D-hexose 6-phosphotransferase antibody
    • FGQTL3 antibody
    • GCK antibody
    • GK antibody
    • GLK antibody
    • Glucokinase antibody
    • Hexokinase D pancreatic isozyme antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HHF3 antibody
    • HK IV antibody
    • HK4 antibody
    • HKIV antibody
    • HXK4_HUMAN antibody
    • HXKP antibody
    • LGLK antibody
    • MODY2 antibody
    see all

Anti-Glucokinase antibody 图像

  • Anti-Glucokinase antibody (ab88056) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg/ml

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa


    Exposure time : 2 minutes
  • ICC/IF image of ab88056 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88056, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.
  • Anti-Glucokinase antibody (ab88056) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa


    Exposure time : 8 minutes

Anti-Glucokinase antibody (ab88056)参考文献

ab88056 has not yet been referenced specifically in any publications.

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